Diaporthe Nitschke, Pyrenomyc. Germ.2: 240 (1870)

MycoBank number: MB 1497; Index Fungorum number: IF 1497; Facesoffungi number: FoF 00146; 450 morphological species (Species Fungorum 2020), 261 species with sequence data.

Type species: Diaporthe eres Nitschke, Pyrenomyc. Germ. 2: 245 (1870)

Nitschke (1867) established Diaporthe to accommodate taxa with stromata in the Sphaeriales. The genus comprised of saprobes, pathogens and endophytes and has a cosmopolitan distribution (Wijayawardene et al. 2020). There are 1067 epithets listed in Index Fungorum (2020), but only 261 have molecular data (Wijayawardene et al. 2020). The phylogenetic tree shows the relationships between the taxa introduced in this study. Diaporthe asheicola, D. eucalyptorum, and D. salsuginosa are illustrated in Hyde et al (2020).

Notes – Diaporthe is the type genus of Diaporthaceae, and it was established by Nitschke (1867) to accommodate taxa with stromata in the Sphaeriales which are often with blackened zones in the substrate, ellipsoid to fusiform ascospores and enclosed, unilocular pycnidia that contain spermatia, stylospores and conidia (Wehmeyer 1933a). Diaporthe species are saprobes, pathogens and endophytes and have a worldwide distribution (Dissanayake et al. 2017). Phomopsis was earlier the asexual morph (Udayanga et al. 2012). However, these genera were linked based on principle of significance and Diaporthe was nominated over Phomopsis to resolve nomenclatural complications (Rossman et al. 2015).

Figure 1 – Phylogram generated from maximum likelihood analysis based on combined ITS, TEF1-α, β-TUB and CAL sequence data. Twenty three strains are included in the combined analyses which comprised 1898 characters (573 characters for ITS, 335 characters for TEF1-α, 505 characters for β-TUB, 485 characters for CAL) after alignment. The evolutionary model GTR+I+G was applied to all the gene regions. Tree topology of the maximum likelihood analysis is similar to the Bayesian analysis. The best RaxML tree with a final likelihood value of -8536.482989 is presented. Estimated base frequencies were as follows: A = 0.223206, C = 0.299928, G = 0.240952, T = 0.235914; substitution rates AC = 0.994418, AG = 2.320262, AT = 0.876368, CG = 0.702354, CT = 3.910746, GT = 1.000000; gamma distribution shape parameter a = 0.541230. Bootstrap support values for ML greater than 75% and Bayesian posterior probabilities greater than 0.90 are given near nodes respectively. The tree is rooted with Diaporthella corylina (CBS 121124). Ex-type strains are in bold and black. The newly generated sequences are indicated in yellow.