Diaporthe breviconidiophora Y.Y. Chen, A.J. Dissan. and Jian K. Liu sp. nov. (Figure e)

MycoBank number: MB; Index Fungorum number: IF; Facesoffungi number: FoF 12616

Etymology: Refers to the short conidiophores.

Holotype: GZAAS 22-0027

Saprobic on decaying wood. Sexual morph: Not observed. Asexual morph: Conidiomata 200–350 μm diam., globose to irregular, erumpent at maturity. Peridium up to 60–150 μm diam., parenchymatous, consisting of 3–4 layers of medium brown textura angularis. Conidiophores 20–45 × 2–2.4 μm, cylindrical, hyaline, smooth, branched, ampulliform, straight to sinuous. Conidiogenous cells up to 0.5–1 μm, phialidic, cylindrical, terminal, with slight tapering towards apex. Paraphyses 20–40 × 1–2 μm, abundant among conidiophores. Alpha conidia (6–)7–8(–9) × 2–3.5 μm ( = 7.5 × 3, n = 30), aseptate, hyaline, smooth, ovate to ellipsoidal, biguttulate, base subtruncate. Beta conidia (17–)19–21(–24) × (1–)1.5–2(–2) μm ( = 20 × 2, n = 30), aseptate, hyaline, smooth, fusiform or hooked, base subtruncate.

Culture characteristics: Colonies covering entire PDA Petri dishes after 10 d at 25 °C producing abundant white aerial mycelium. Reverse white, turning to grey in centre.

Material examined: China, Guizhou Province, Xingyi city, saprobic on decaying woody host, June 2020, Y. Y. Chen (GZAAS 22-0027, holotype); ex-type living culture CGMCC = GZCC 22-0026; ibid, DuShan county, saprobic on decaying woody host, May 2020, Y. Y. Chen (GZAAS 22-0050, paratype), living culture GZCC 22-0049.

Notes: The phylogenetic results showed that two isolates of Diaporthe breviconidiophora clustered within the D. citrichinensis species complex forming a distinct lineage (Figures 2, 18) with maximum support (ML/MP/BI = 100/100/1.0). Diaporthe breviconidiophora can be distinguished from D. citrichinensis in the concatenated alignment by 13/512 in ITS, 8/413 in tef, 14/453 in tub, 12/506 in cal and 5/515 in his. Morphologically, D. breviconidiophora differs from D. citrichinensis in having shorter beta conidia (Huang et al. 2013).

Figure e. Diaporthe breviconidiophora (GZAAS 22-0027, holotype). a, b Conidiomata on host surface. c Section of conidiomata. d Alpha conidia. e Alpha conidia stained in methylene blue. f Beta conidia attached to conidiophores. g Beta conidia. h 10 days old culture on PDA from above. i 10 days old culture on PDA from reverse. Scale bars: c = 100 μm; d–g = 20 μm.