Tremateia halophila Kohlm., Volkm.-Kohlm. & O.E. Erikss., Bot. Mar. 38(2): 166 (1995).

Index Fungorum number: IF 414298; MycoBank number: MB 414298; Facesoffungi number: FoF 00062

Saprobic on dead wood in terrestrial or marine. Sexual state Ascomata 225–320 × 330−410 µm (= 260 × 355 µm, n = 10), solitary, scattered, brown to black, immersed in the host tissue, globose to subglobose. Peridium 15–25 µm diam, composed of two layers, outer layer composed of irregular, thick-walled, brown cells of textura angularis. The inner layer composed of hyaline, larger cells of textura angularis. Hamathecium 2–25 µm dense of transverse septa, long, colourless, branched, encircled ascus. Asci 120–170× 24−30 µm (= 150 × 26 µm, n = 20), 4-8-spored, bitunicate, clavate to broadly clavate, short pedicellate, thin-walled, with ascospores arranged in a cluster. Ascospores 25–42 × 12−20 µm (= 30 × 16 µm, n = 20), uniseriate or biseriate, ellipsoid to fusiform, light brown to brown, muriform, constricted at the middle septum, cells 2-layered, sometimes rounded at both sides with a 15–25 µm wide, large uniform a sheath. Asexual state not observed.

Material examinedNorth Carolina: Broad Creek, On senescent culms of Juncus roemerianus, 28th November 1993, J. J. Kohlmeyer 5522 (NY 01353617-01353627, holotype).

Fig. 1 Tremateia halophila (holotype). a, b, d Vertical hand section of ascomata. c, e Vertical hand section of ostiole and peridium. f–j Asci with muriform ascospores. k Hamathecium. l–q Fusiform, light brown to brown, muriform ascospores. Scale bars: a–d =50 µm, c, e = 20µm, f–k = 10 µm, l–k = 5 µm.

Notes Tremateia was introduced as a facultative marine genus and typified with T. halophila (Kohlmeyer et al. 1995). The genus is characterized by depressed globose, immersed ascomata, numerous and cellular pseudoparaphyses, fissitunicate and clavate asci, ellipsoid muriform ascospores, and a Phoma-like asexual stage (Kohlmeyer et al. 1995). Based on these morphological characters, Kohlmeyer et al. 1995 transferred Tremateia to Pleosporaceae. Furthermore Kohlmeyer et al. 1995 suggested that Lewia M.E. Barr & E.G. Simmons and Diademosa Shoemaker & C.E. Babc. are superficially similar to Tremateia. DNA sequences-based phylogenies of recent literatures and also in the present study have shown that T. halophila nested in Montagnulaceae sister to Bimuria novae-zelandiae. (Schoch et al. 2009; Suetrong et al. 2009), thus familial status of Tremateia is verified.