Letendraea brasiliensis (Dianese et al.), Phookamsak, Ariyawansa & K.D. Hyde comb. nov.,
Basionym: Wilmia brasiliensisDianeseet al., Mycologia 93(5): 1014 (2001).
Index Fungorum number: IF 550698; MycoBank number: MB 550698; Facesoffungi number: FoF 00046
Parasitic on Memora pedunculata. Sexual state: Ascomata 140–200 × 90–125 µm, scattered, rarely clustered, immersed to erumpent through host surface, globose to sub globose, brown to dark brown, central ostiole. Ostiole papillate with elongated periphyses. Peridium 6–21 µm wide, composed of 2–5 layers, thin-walled with equal thickness, small, flattened pseudoparenchymatous, brown to dark brown cells of textura angularis to textura prismatica. Hamathecium dense of 1.5–3 µm wide, cellular pseudoparaphyses, unbranched, not-constricted at the septa, hyaline, embedded in a mucilaginous matrix. Asci 70–90(-98) × (11-)13–15(-17) µm (= 82.9 × 13.8 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate, short-pedicellate, apically rounded with indistinctly ocular chamber. Ascospores (18-)20–24 × 6–7(-7.5) µm (= 21.9 × 6.9 μm, n = 30), overlapping, 1–2-seriate, didymosporous, fusiform with obtuse ends, brown, 1-septate, constricted at the septum, smooth-walled, with indistinct appendage at the ends, surrounded by mucilaginous sheath. Asexual state: cohabit with the sexual state on leaf lesions. Conidiomata 80–220 µm high, 100–165 µm diam., pycnidial, scattered to clustered, solitary, immersed to erumpent through host surface as black dots, visible as slightly raised or bumped, small, pale brown to brown spots on host surface, uniloculate, globose to sub globose, brown to dark brown, central ostiole, papillate with large periphyses. Conidiomata walls 8–14 µm wide, composed of 2–5 layers, thin-walled cells with equal thickness, small, flattened pseudoparenchymatous cells of textura angularis to textura prismatica, brown to dark brown. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 5–10 × (4.5-) 5–7(-10) µm (x̄ = 7 × 6.4 μm, n = 20), enteroblastic, phialidic, ampulliform to lageniform, hyaline, smooth-walled, aseptate. Conidia (12-)14–20(-26.5) × 5–7 µm (x̄ = 15.9 × 6.4 μm, n = 30), oblong to obovoid or ellipsoidal with rounded or obtuse ends, hyaline, indistinctly 1-distoseptate, rough-walled.
Material examined – BRAZIL, Distrito Federal Vargem Bonita, Fazenda Áqua Limpa, on leaves of Memora pedunculata Vell. (Bignoniaceae), 18 May 1995, C.A. Inácio (UB 8438, holotype of Wilmia brasiliensis).
Fig. 1 Letendraea brasiliensis (UB 8438, holotype of Wilmia brasiliensis). a Herbarium label and specimens. b Ascomata on host surface. c Section through ascomata stained by cotton blue reagent. d Section through peridium. e Pseudoparaphyses stained by Congo red reagent. f–h Ascospores. i–k Asci. l Section through conidiomata stained by cotton blue reagent. m Conidiogenous cells. n Conidia. o–q Conidia. Scale bars: c=100 µm, l=50 µm, d–k=20 µm, m= 10 µm, f–n=5 µm, o–q=2 µm.