Tubeufia javanica Penz. & Sacc., Malpighia 11(11–12): 517 (1898).
Holotype: INDONESIA, Java, Tjibodas, on withered culm of Bambusa emarcidis, 2 Mar 1987 (PAD)
Epitype designated here: MFLU 13–0371 Saprobic on culms or sheaths of bamboo. Sexual state: Ascomata 376–505(−550) μm high×(200-)224–260(−280) μm diam., superficial, seated on a subiculum, solitary, sometimes gregarious, oblong, subclavate to obclavate, creamwhite to yellowish when young, pale brown to brown when mature, apex rounded, base narrow, brown to dark brown, collapsing when dry, compressed subiculum hyphae, 2.5– 5μm wide, partially branched, septate, dark brown, and irregular. Peridium 15–25μm wide, comprising light brown cells of textura angularis, and small textura prismatica subhyaline cells inwardly. Hamathecium comprising numerous, 1–2μm wide, filiform, septate, branched, hyaline pseudoparaphyses. Asci ( 1 4 0 – ) 164 – 221 ( − 2 3 0 ) × 14– 1 8 ( − 22 ) μm (x¼ 194 16μm , n=20), 8-spored, bitunicate, fissitunicate, cylindrical, short pedicellate, apically rounded, with an ocular chamber. Ascospores (95-)100.5–117(−120)×4–5μm (x¼ 109 4:5μm , n=20), fasciculate, broad filiform, cylindrical to long subfusiform, elongate, ends rounded, 14–17- septate, not constricted at septa, hyaline to pale yellow or brown, smooth-walled. Asexual state: Unknown. Cultural characteristics: Ascospores germinating on PDA within 24 h and germtubes produced from both ends. Colonies growing slowly on PDA, reaching 4.5mmin 2 weeks at 28C, effuse, velvety to hairy, edge fimbriate, olive to olive brown, dark brown in PDA media. Mycelium superficial and partially immersed, branched, septate, pale brown to olivaceous brown, smooth, asexual spores not formed within 60 days.
Material examined: THAILAND, Chiang Rai, Muang, Khun Korn Waterfall, on dead culm of bamboo Kunth ex Dumort (Poaceae), 31 July 2012, Dong-Qin Dai, DDQ00239 (MFLU 13–0371, epitype of Tubeufia javanica designated here; PDD 104450, iso-epitype), ex-type living culture = MFLUCC 12–0545 = ICMP 20067; Chiang Rai, Muang, Khun Korn Waterfall, on dead culm-sheath of bamboo, 21 July 2013, S. Boonmee KK-11 (MFLU 14–0222), living culture = MFLUCC 14–0438.
Notes: We requested the type speciman from PAD and BO but this is not present in either herbarium and therefore must be presumed lost. We therefore designate our fresh collection as epitype to stabilize applications of the genus and species. The epitype is representative of the iconotype and description provided in the protologue, and the location in Asia and bamboo host are also appropriate for epitypification (Penzig and Saccardo 1904; Boonmee et al. 2011).
Fig. 1 Tubeufia javanica (MFLU 13–0371, epitype). a, b Ascomata formed on natural substrate. c Close up of pale reddish-brown ostiole. d Side view of ascoma located on a subiculum. e L.S. of ascoma. f Peridium. g Pseudoparaphyses. h–j Asci. k Ruptered ascus. l–o Ascospores. p Germinating ascospore. q Dark brown colonies on PDA. r, s Growth on plant substrates and aerial hyphae in culture. Scale bars: a = 500μm, b–c = 200μm, d–e, h–k = 100μm, f = 40μm, e = 5μm, l–p = 50, q–r = 10mm, s = 20μm