Colletotrichum quinquefoliae Jayawardena, Bulgakov & K.D. Hyde, sp. nov., Index Fungorum number: IF 551745

Etymology: The specific epithet quinquefoliae is named after the host Parthenocissus quinquefolia (L.) Planch. from
which the taxon was collected.

Saprobic and weak pathogen on dying and dead leafstalks, twigs and tendrils of Parthenocissus quinquefolia. Sexual morph Undetermined. Asexual morph Conidiomata 267– 517 μm (x = 410 μm, n = 10) diam., black, acervulus, oval, solitory, gregarious. Setae straight or ± bent, abundant, dark brown, becoming paler towards the apex, opaque, smoothwalled, septa difficult to distinguish, 1–5-septate, 58–258μm long, base cylindrical, 6.8–10.5 μm diam., tip somewhat acute. Conidiophores medium brown, smooth–walled, simple, to 35 μm long. Conidiogenous cells 7.3–12.8 × 1.4–3.3 μm (x = 8.5 × 2.5 μm, n = 20), hyaline to pale brown, smooth–walled, cyllindrical to slighty inflated, opening 0.5–1 μm diam., collarette or periclinal thickening not observed. Conidia 5.9–15.8 × 2.2–5.2 μm (x = 9.9 × 3.3 μm, n = 40), L/Wratio 3.0, hyaline, smooth or verruculose, aseptate, curved, both sides gradually tapering towards the round to slightly acute apex and base, guttulate.

Material examined: RUSSIA, Rostov region, Rostov-on- Don city, Botanical Garden of Southern Federal University,
Higher Park, underwood, on Parthenocissus quinquefolia (Vitaceae), 5 March 2014, T.S. Bulgakov (MFLU 14–
0626, holotype), (isotype in GZAAS, under the code of GZAAS 15–0101).

Notes: Colletotrichum dematium species complex is mainly characterized by having curved conidia (Dammet al. 2009). Colletotrichum quinquefoliae falls within the Colletotrichum dematium species complex and forms a separate clade which is supported by 100 % bootstrap value and 1.00 Bayesian posterior probability . This species differ from C. menispermi in having larger conidiomata, 1–5-septate, long setae, with a larger base and conidia with an acute base. This species differs from C. circinans and C. spinaceae in having longer setae with 1–5 septa and simple conidiophores.

Fig. 1 Phylogram generated from parsimony analysis based on combined ITS, GADPH, CHS, ACT and β-tubulin sequence data of Colletotrichum. Parsimony bootstrap support values greater than 50 % are indicated above or below the nodes, and branches with Bayesian posterior probabilities greater than 0.95 are given in bold. The ex-type strains are in bold; the new isolates are in blue. The tree is rooted with Monilochaetes infuscans CBS 869.96.

Fig 2. Colletotrichum quinquefoliae (holotype) a Conidiomata on host b Black acervuli with setae c Acute tip of the setae d Base of the setae e Seta f Conidiophores g Conidiogenous cell h Conidium Scale bars: b = 200 μm, c = 50 μm, = 5 μm, = 150 μm, f = 20 μm, g–i = 5 μm.