Amylosporus guaraniticus Campi & Robledo 

MycoBank number MB821985 Facesoffungi number: FoF 03568

Etymology— Guaraniticus refers to the area where Guaraní indigenous ethnicity inhabited, mainly Eastern Paraguay and NE Argentina.

Holotypus. Paraguay, San Lorenzo, University Campus, 03.III.2016, leg. M. Campi 106 (FACEN 3422).

Basidiomata annual, sessile, imbricate, semicircular, broadly adhered to the surface, 23 × 12 × 5 cm, fleshy and soft when fresh, corky when dry. Pileal surface glabrous to velvety, soft when fresh, whitish to pale brown, with chestnuts areas towards the base which become dark brown or blackish at maturity. Margin sterile, round, whitish to cream when fresh, acute and light brown when dry. Pores angular 2–3 per mm, dissepiments entire occasionally lacerate, pore surface whitish when fresh becoming cream to light brown when dry. Context homogeneous, fleshy, white to cream when fresh, corky and light brown when dry, up to 42 mm. Tube layer concolorous with the context up to 10 mm thick. Hyphal system dimitic, generative hyphae thin-walled, 4–6.5 μm diam; the context composed of with generative hyphae 6–14 μm diam., with simple septa and double or multiple clamp connections, gloeopleurous hyphae present. Skeletal hyphae restricted to the tubes, thick-walled, 3–8 μm diam, branched, flexuous, IKI–. Basidia clavate, 10– 25 × 5–8 μm, with four sterigmata 2–6 μm long. Basidiospores ellipsoid, (3.8)4–5.5(6.5) × (2.5)3–4 (4.5) μm, X=4.6 × 3.4 μm, Q= 1.4, N=2, hyaline, thin- to slightly thick-walled, finely asperulate, with a central guttule, strongly amyloid.

Known distribution – So far know from type locality. Records of A. campbellii in South America must be reviewed in order to confirm their identity, see discussion.

Material examined – Paraguay, Central Department, San Lorenzo city, Universidad Nacional de Asunción, Facultad de Ciencias Químicas FCQ, inside a sump, 25°23´21.55´´ S 57°10´56.1´´ W, 245 m a.s.l., 03 March 2016, M. Campi, 106 (FACEN 003422, HOLOTYPE). Ibid, Facultad de Arquitectura, Diseño y Arte, coming out from between the brick board against the floor , 25°33´38.47´´ S 57°51´64.04´´ W 245 m a.s.l., 2 Feb 2016, M. Campi, 105 (FACEN 003421).

Strict consensus tree from Bayesian inference of the combined ITS-LSU dataset illustrating the phylogenetic relationships of Amylosporus within Wrightoporiaceae. The new species specimens from which new sequences were obtained in this work are in boldface. T= type specimen.

Amylosporus guaraniticus (FACEN 003422, HOLOTYPE) macroscopical features. A-B general view inside a sump, C close up of pores, D longitudinal section showing context ant tube layer

Amylosporus guaraniticus (FACEN 003422, HOLOTYPE) microscopical features A-B multiple clamped generative hyphae. C simple septate generative hyphae. D Basidia. E Basidiospores in KOH + Floxine, showing refringent central guttula. F Basidiospores in Melzer´s reagent.