Calonectria eucalypti L. Lombard, M.J. Wingf. & Crous, Stud. Mycol. 66: 47 (2010) Fig. 3
MycoBank number: MB 515530; Index Fungorum number: IF 515530; Facesoffungi number: FoF 11340;
Micromorphology: Associated with leaf spot of Callistemon rigidus. Telemorph: not observed. Anamorph: Macroconidiophores 15–45 × 4–8 μm, septate, hyaline. Primary branches of conidiogenous apparatus; secondary branches aseptate, 10–25 × 4–8 μm; tertiary branches 10–20 × 3–8 μm; each terminal branch producing 2–6 phialides, 8–20 × 3–6 μm. Vesicles 4–8 (–10) μm diam, clavate to broadly clavate. Macroconidia (65–)70–80(–87) × (5–)6 μm (x̄ = 75 × 6.5 μm, n=50), cylindrical, straight, 1–3 septate. Megaconidia and microconidia not observed.
Culture characteristics: Colonies on MEA at 25 ℃, growth rate 8.7 mm/d (n = 5), circular, producing abundant white aerial mycelium; reverse brown.
Material examined: China, Guangdong province, Guangzhou city, Callistemon rigidus R. Br., (Myrtaceae), 10th November 2020 (Dry culture ZHKU 21-0010), and living culture, ZHKUCC 21-0013.
Notes: In the phylogenetic tree, our isolates were grouped with Calonectria eucalypti with 77 % in ML, and 0.97 in BYPP support. The macroconidia of the isolate belonging to this study (x̄ = 75 × 6.5 μm) are similar to the size of the Ca. eucalypti (x̄ = 72 × 6 μm) described by Lombard et al. (2010). In addition, the vesicles shape and dimension of our isolate are similar to Ca. eucalypti (Lombard et al., 2010). We introduce our isolates from Callistemon rigidus as an anamorph of Ca. eucalypti based on phylogenetic analyses and asexual morphological characteristics.Ca. eucalypti from Callistemon rigidus.
Figure 3. Calonectria eucalypti (ZHKUCC 21-0013: New host record) a–b: Culture characteristics on MEA after 7 days (a, upper view; b, reverse view); d: Macroconidiophores; c, e: Conidiogenous apparatus; f–i: Vesicles; j–k: Macroconidia; Scale bars: c–k = 10 μm.