Coniochaetales Huhndorf, A.N. Mill. & F.A. Fernández, Mycologia 96(2): 378 (2004)

Index Fungorum number: IF 501515; MycoBank number: MB 501515Facesoffungi number: FoF 01379;

This order consists of two families, Coniochaetaceae representing the genera Barrina and Coniochaeta and Cordanaceae, which accommodates Cordana. Cordanaceae was previously classified in Cordanales, however, based on molecular clock evidence, Hongsanan et al. (2017) treated Cordanales as a synonym of Coniochaetales. The phylogenetic tree presented here supports this synonymy (Fig. 9). The divergence time for Coniochaetales has been estimated as 131 MYA (Fig. 2). Currently, there are two families and five genera in this order.

Figure 2 – The maximum clade credibility (MCC) tree, using the same dataset from Fig. 1. This analysis was performed in BEAST v1.10.2. The crown age of Sordariomycetes was set with Normal distribution, mean = 250, SD = 30, with 97.5% of CI = 308.8 MYA, and crown age of Dothideomycetes with Normal distribution mean = 360, SD = 20, with 97.5% of CI = 399 MYA. The substitution models were selected based on jModeltest2.1.1; GTR+I+G for LSU, rpb2 and SSU, and TrN+I+G for tef1 (the model TrN is not available in BEAUti 1.10.2, thus we used TN93). Lognormal distribution of rates was used during the analyses with uncorrelated relaxed clock model. The Yule process tree prior was used to model the speciation of nodes in the topology with a randomly generated starting tree. The analyses were performed for 100 million generations, with sampling parameters every 10000 generations. The effective sample sizes were checked in Tracer v.1.6 and the acceptable values are higher than 200. The first 20% representing the burn-in phase were discarded and the remaining trees were combined in LogCombiner 1.10.2., summarized data and estimated in TreeAnnotator 1.10.2. Bars correspond to the 95% highest posterior density (HPD) intervals. The scale axis shows divergence times as millions of years ago (MYA).

Figure 2 – Continued.

Figure 2 – Continued.

Figure 9 – Phylogram generated from maximum likelihood analysis based on combined LSU and ITS sequence data of Coniochaetales. Forty-two strains are included in the combined analyses which comprised 2640 characters (521 characters for LSU, 567 characters for ITS) after alignment. Chaetosphaeria garethjonesii (MFLUCC 15-1012) (Chaetosphaeriaceae, Chaetosphaeriales) is used as outgroup taxon. Single gene analyses were carried out and the phylogenies were similar in topology and clade stability. Tree topology of the maximum likelihood analysis is similar to the Bayesian analysis. The best RaxML tree with a final likelihood value of – 6488.978861 is presented. Estimated base frequencies were as follows: A = 0.249647, C = 0.238753, G = 0.279808, T = 0.231793; substitution rates AC = 1.587009, AG = 2.017143, AT = 1.817611, CG = 1.386630, CT = 7.265255, GT = 1.0000; gamma distribution shape parameter a = 0.484696. Bootstrap support values for MP and ML greater than 50% and Bayesian posterior probabilities greater than 0.9 are given near the nodes. Ex-type strains are in bold. The newly generated sequences are indicated in blue.


Coniochaetales genera incertae sedis