Colletotrichum hymenocallidis Yan L. Yang, Zuo Y. Liu, K.D. Hyde & L. Cai, in Yang, Liu, Cai, Hyde, Yu & McKenzie, Fungal Diversity 39: 138 (2009)

Index Fungorum number: IF 515281; Facesoffungi number: FoF 00014

Etymology – hymenocallidis, in reference to the host Hymenocallis americana.
In agaro decoct tuberosum post 6 dies colonae est 6-6.4 cm diam, albo vel pallid griseae cum orbis, densus, reversum pallid griseae vel pallens nigri, margine albo. Sclerotia absentia. setae absentia. Conidia in persicinum massa, 14–20 × 5-6.5 µm ( x = 15.9 ± 1.1 × 5.1 ± 0.40, n = 100), aseptata, laevia, hyalina, fusiformia, recta cum unus vel duo guttulatae, interdum ad centralis anqustus, ad apicem obtusa. Appressoria atrobrunnea, ovata, interdum irregularis, margine pluries totus, interdum crenate vel lobulata, 7-11 × 5-7.5 µm ( x = 8.5 ± 0.9 × 6.6 ± 0.6, n = 40).

Habitat – in foliis Hymenocallis americanis

Holotypus – Cultura (CSSN2), isolata e foliis morbo affectis Hymenocallis americana, Nanning, China, 2009.

Paratypus – Cultura (CSSN3), isolata e foliis morbo affectis Hymenocallis americana, Nanning, China, 2009.

Leaf lesions elliptical or circular, reddish-brown (Fig. 6E). On host, acervuli subepidermal, disrupting the outer epidermal cell wall of the host, setae absent, with salmon-pink conidia masses (Fig. 5A). Conidiophores hyaline, 1-2 celled, not branching or branching at the base, 9-20 × 3-4.5 µm ( x = 14.7 ± 3.1 × 3.8 ± 0.5, n = 20) (Fig.5D). Conidia 9.5-15 × 3.5-5.5 µm ( x = 13.1 ± 1.4 × 4.8 ± 0.4, n = 50), one-celled, smooth-walled, hyaline, straight, sometimes slightly curved, fusiform, gradually tapered at the ends (Figs 5E, F).

Culture characters – Colonies on PDA attaining 6-6.4 cm in diam. ( x = 6.2 ± 4.4, n = 10) in 6 days at 25°C, growth rate 8.8-11 mm per day ( x = 9.9 ± 0.8, n = 10); at first white, with ageing, becoming pale grey from the centre with concentric zones, dense, reverse pale white at first, becoming greenish black from the centre with age, margin white (Figs 5B, C). Sclerotia absent. Setae absent. Conidia formed in orange-pink conidial masses, 14-20 × 5-6.5 µm ( x = 15.9 ± 1.1 × 5.1 ± 0.40, n = 100), one-celled, smooth-walled, hyaline, fusiform, straight, guttulate, occasionally constricted at centre, obtuse at the ends (Figs 5G-I). Appressoria dark brown, ovate, irregular, margin entire, or crenate to lobed, occasionally forming secondary appressorium from first appressorium, but not complex (Figs 5J-L), 7-11 × 5-7.5 µm ( x = 8.5 ± 0.9 × 6.6 ± 0.6, n = 40). Teleomorph: not produced in culture.

Holotype – China, Guangxi Province, Nanning, on leaf spot of Hymenocallis americana, 19 June 2008, Y. L. Yang (GZAAS 080001; ex-holotype living culture CSSN2, CBS 125378).

Known host and distribution – Hymenocallis americana, Guangxi, China.

Additional specimens examined – China, Guangxi Province, Nanning, on leaf spot of Hymenocallis americana, 19 June 2008, Y.L. Yang (GZAAS 080002, ex-paratype living culture CSSN3, CBS 125379).

Fig. 1. Colletotrichum hymenocallidis (from holotype). A. Acervuli on leaf of Hymenocallis americana. B, C. Colony on PDA after 7 days, upper B and reverse C. D. Conidiophores. E and F. Conidia from conidial masses on leaf of H. americana. G-I. Conidia from conidial masses from culture. J-L. Appressoria (arrows indicate secondary appressoria). Scale bars: A = 200 µm; D-L = 10 µm.