Setoapiospora thailandica Mapook & K.D. Hyde, sp. nov.

MycoBank number: MB 556906; Index Fungorum number: IF 556906; Facesoffungi number: FoF 06794; Fig. 86

Etymology: Name reflects the country from where this species was collected, Thailand.

Holotype: MFLU 20-0049

Saprobic on dead branches of wood. Sexual morph Ascomata  270–290  µm   high × 390–415  µm   diam. ( x̄ = 280.5 × 400 µm, n = 5), superficial to semi-immersed, solitary or scattered, carbonaceous, solitary or scattered, appearing as dark brown to black spots, with a poorly developed basal layer and an irregular margin. Ostiole central, with external dark brown setae. Peridium 25–50(–60) µm wide, comprising dark brown cells of textura epidermoidea. Hamathecium comprising 1–2 µm wide, cylindrical to filiform, septate, pseudoparaphyses. Asci 85–160 × 13–24 µm ( x̄ = 123 × 17 µm, n = 20), 8-spored, bitunicate, cylindrical, pedicellate, straight or slightly curved, with an ocular chamber. Ascospores 20–27 × 10–13 µm (x̄ = 25 × 11 µm, n = 25), 1-seriate, ellipsoid to broadly fusiform, hyaline, 1-septate, constricted at the septum, with a small lower cell and a large upper cell, widest at the centre and tapering towards ends, granular. Asexual morph Undetermined.

Culture characteristics: Ascospores germinating on MEA within 24 h at room temperature and germ tubes produced from the apex. Colonies on MEA irregular, initially mycelium white, slightly flattened, filamentous, cultures grayish to light brown in old, flattened on surface, pale brown to brown in reverse from the centre of the colony, creamy white at margin

Material examined: THAILAND, Chiang Rai Province, Huai Kang Pla waterfall, on dead branches of wood, 19 June 2015, A. Mapook, HKP1 (MFLU 20-0049, holotype), ex- type culture MFLUCC 17-1426.

GenBank numbers: ITS = MN638862, LSU =MN638847, SSU = MN638851, TEF1-α = MN648731.

Notes: In a BLASTn search of NCBI GenBank, the closest matches of the ITS sequence of Setoapiospora  thailandica (MFLUCC 17-1426, ex-holotype) with 81.36% similarity, was to Mycoleptodiscus terrestris (strain IMI 159038, MK487755). The closest matches with the LSU sequence with 93.74% similarity, was to Mycoleptodiscus terrestris (strain CBS 231.53, MK487730), while the closest matches with the SSU sequences with 98.52% similarity, was to Neocochlearomyces chromolaenae (strain BCC 68250, NG_065766).

Fig. 86 Setoapiospora thailandica (MFLU  20-0049,  holotype). a, b Appearance of ascomata on substrate. c Squash mounts show- ing ascomata. d Section through of ascoma. e Setae. f Peridium. g Pseudoparaphyses. h, i asci. j–m Ascospores. Scale bars: a = 500 µm, b = 200 µm, d = 100 µm, c, h, i = 50 µm, e, f, j–m = 10 µm, g = 5 µm