Pseudomurilentithecium camporesii Mapook & K.D. Hyde, sp. nov.

MycoBank number: MB 556903; Index Fungorum number: IF 556903; Facesoffungi number: FoF 06792; Fig. 42

Etymology: Named in honour of the collector, Erio Camporesi.

Holotype: MFLU 20-0047

Saprobic on dead aerial stem of Vicia sp. Sexual morph Ascomata (90–)130–145 µm high, 140–160 µm diam. ( x̄ = 123 × 154 µm, n = 5), immersed, solitary or scattered, coriaceous, subglobose to globose, dark brown to black, ostiolar neck protruding. Peridium 14–20 µm wide, 2–3 layers, comprising dark brown cells of textura angularis. Hamathecium comprising 2.5–5(–7) µm wide, cylindrical, septate, branching pseudoparaphyses. Asci 90–115 × 16–22 µm ( x̄ = 104 × 19 µm, n = 10), 8-spored, bitunicate, cylindric- clavate, straight or slightly curved, apically rounded, pedicel- late. Ascospores (20–)27–32 × 8–12 µm (x̄ = 28.5 × 10 µm, n = 40), overlapping, 1–2 seriate, initially hyaline to pale yellow, 1-septate when immature, becoming golden-brown to brown at maturity, ellipsoid to broadly fusiform, muri- form, 3–7-transversely septate, with 1–2 longitudinal septa ,  slightly constricted at the central septum, straight or slightly curved, surrounded by hyaline gelatinous sheath clearly observed when mounted in Indian ink. Asexual morph Undetermined.

Material examined: ITALY, Province of Forlì-Cesena [FC], Monte Coronaro-Verghereto, on dead aerial stem of Vicia sp. (Fabaceae), 26 July 2014, E. Camporesi, IT 2018 (MFLU 20-0047, holotype), ex-type living culture, MFLUCC 14-1118.

GenBank numbers: ITS =MN638861, LSU =MN638846, SSU = MN638850, TEF1-α = MN648730.

Notes: In a BLASTn search of NCBI GenBank, the closest matches of the ITS sequence of Pseudomurilentithecium camporesii (MFLUCC 14-1118, ex-holotype) with 90.38% similarity, was to Keissleriella cladophila (strain CBS 104.55, MH857391). The closest matches of the LSU sequence with 94.57% similarity, was to Parathyridaria ramulicola Jaklitsch, Fourn. & Voglmayr (strain MF4, KX650564), 94.65% similarity, was to Bimuria novaezelandiae D. Hawksw., Chea & Sheridan (strain CBS 107.79, NG_058623), and 94.45% similarity, was to Keissleriella cladophila (strain CBS 104.55, GU205221). The closest matches with the SSU sequences with 90.98% similarity, was to Darksidea alpha D.G. Knapp et al. (strain CBS 135628, KP184054). The closest matches of the TEF1-α sequences with 91.35% similarity, was with Misturatosphaeria sp. (strain MFLUCC 10-0126, KR075170), while the closest matches of the RPB2 sequences with 78.79% similarity, was to Murilentithecium clematidis Wanas. et al. (strain MFLUCC 14-0562, KM454447). In the phylogenetic analyses (Fig. 43), P. camporesii forms a basal clade to Poaceascoma.

Fig. 42 Pseudomurilentithecium camporesii (MFLU 20-0047, holo- type). a, b Appearance of ascomata on substrate. c Section through ascoma. d Peridium. e Pseudoparaphyses. f, g asci. hj Ascospores. k Ascospores with gelatinous sheath in Indian ink. Scale bars: a, b = 200 µm, c, f, g = 50 µm, d = 20 µm, e, hk = 10 µm

Fig. 43 Phylogram generated from maximum likelihood analysis based on combined dataset of LSU, ITS, SSU, TEF1-α and RPB2 sequence data. Seventy-seven strains are included in the combined sequence analysis, which comprise 4008 characters with gaps. Tree topology of the ML analysis was similar to the BI. The best scoring RAxML tree with a final likelihood value of − 18684.534541 is pre- sented. The matrix had 1199 distinct alignment patterns, with 37% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.241414, C = 0.246131, G = 0.273783, T = 0.238671;

substitution rates: AC = 1.203261, AG = 1.960685, AT = 1.267413, CG = 1.440469, CT = 7.447474, GT = 1.000000; gamma distribution shape parameter α = 0.166789. Bootstrap support values for ML equal to or greater than 60% and BYPP equal to or greater than 0.90 are given above the nodes. Newly generated sequences are in blue bold and type strains are in black bold. Bambusicola bambusae (MFLUCC 11-0614) and B. irregulispora (MFLUCC 11-0437) are used as out- group taxa