Nigrospora gorlenkoana Novobr., Nov. sist. Niz. Rast. 9:180 (1972)
Index Fungorum number: IF 516929; Facesoffungi number: FoF 06595
Pathogenic or saprobic on leaves of Cirsium setosum (Willd.) Besser ex M.Bieb (Asteraceae). Asexual morph: Hyphae smooth, branched, septate, and hyaline. Conidiophores mostly reduced to conidiogenous cells. Conidiogenous cells 6.9–10 × 4.2–8 μm diam. (x̄ = 8.4 × 6 μm , n = 30), monoblastic, solitary, discrete, determinate, doliiform to ampulliform, and pale brown. Conidia 10.3–14 × 13.3–17.2 μm diam. (x̄ = 12.5 × 15.2 μm, n = 50), solitary, globose or oblate, dark brown to black, shiny, sparse, discrete on aerial mycelia, and smooth-walled. Sexual morph: Undetermined.
Culture characteristics – Colonies on PDA reach 9 cm diam. after 5 d at 25 C, circular shaped, entire margined, floccose with aerial mycelium, surface initially white, turning grayish when mature and reverse initially white, turning smoke gray when mature.
Material examined – China, Shandong Peninsula, on living leaves of Cirsium setosum, 07 October 2017, Yuanyuan Hao (JZBH 3230001), living culture JZB 3230001, and KUMCC 19-0222.
Leaf spot symptoms – Leaf spots irregularly scattered and composed of a dark brown circular outer ring with a light brown inner ring, margined by apparently healthy leaf tissues.
Notes – Based on the phylogenetic analysis of combined ITS, TEF, and TUB2 sequence data of Nigrospora species (Figure 1), our strain Nigrospora gorlenkoana (JZB 3230001) clustered with the ex-type strain of N. gorlenkoana (CBS 480.73) with strong bootstrap support and Bayesian probabilities (100% ML, 100% MP, and 1.00 BYPP) (Figure 1). The base pair difference comparison of ITS, TEF, and TUB2 gene regions between our strain (JZB 3230001) and ex-isotype strain of N. gorlenkoana (CBS 480.73) reveals less than 1% difference, and the two specimens share similar morphological characters confirming both strains are conspecific. In contrast to the ex-type strain (CBS 480.73), an equatorial slit on conidia was not observed in our strain (JZB 3230001). Nigrospora gorlenkoana has not frequently been identified as a plant pathogen, and it was previously reported to be isolated from leaves and fruits of Vitis vinifera. This is the first report of Nigrospora gorlenkoana from Cirsium setosum.
![](https://www.facesoffungi.org/wp-content/uploads/2023/08/Nigrospora-203x300.jpg)
Figure 1. Multilocus phylogenetic tree based on the combined ITS, TEF1, and TUB2 sequences alignment generated from a maximum likelihood phylogenetic analysis. Bootstrap support values for ML, MP (> 70%), and posterior probabilities (> 0.9) are given at the nodes (ML/MP/PP). The tree is rooted with Arthrinium malaysianum (CBS 102053) and Arthrinium obovatum (LC 4940). (* indicates the ex-type isolates)
![](https://www.facesoffungi.org/wp-content/uploads/2023/08/Nigrospora-gorlenkoana-300x196.jpg)
Figure 2. Nigrospora gorlenkoana (JZB 3230001). (a and b) Appearance of leaf spots on the host substrate; (c and d) Upper view (c) and reverse view (d) of culture on PDA; (e) Conidia on aerial mycelia on PDA; (f) Mature conidia; (g–i) Mature conidia attached to conidiogenous cells. Scale bars f, g = 20 μm, h, i = 10 μm.