Muriphila oklahomaensis Jurjević, Čmoková & Hubka, in Crous et al., Persoonia 44: 423 (2020)

MycoBank number: MB 834486; Index Fungorum number: IF 834486; Facesoffungi number: FoF 11390;

Etymology. Name refers to the state in the USA where it was collected, Oklahoma.

Micromorphology (on MEA): Hyphae dark olivaceous, moderately thick, with smooth occasionally verrucous walls. Hyphal cells rectangular to sub-spherical, occasionally asymmetrical, 8 – 25(– 45) × 4 –11 µm diam, occasionally 1(– 2) septa, at maturity hyphae fall apart into separate cells. Sexual morph unknown.

Culture characteristics: (in darkness, 25 °C after 21 d): Colonies on malt extract (Oxoid) agar (MEA) 13 –15 mm diam, dark olivaceous to black, velvety, abruptly rising, 5 –7 mm high, radially moderate deep to deep sulcate, crateriform; aerial mycelia absent; reverse black. Colonies on Czapek yeast autolysate agar (CYA) 6 – 8 mm diam, dark olivaceous black to black, smooth, abruptly rising approximately 3 mm high, radially moderate deep sulcate near wrinkled; reverse black. Colonies on potato dextrose agar (PDA) 13 –14 mm diam, dark olivaceous black to black, velvety, approximately 3 – 4 mm high, radially moderate deep to deep sulcate; aerial mycelium absent; reverse black. Colonies on oatmeal agar (OA) 12 –14 mm diam, black, smooth, abruptly rising approximately 3 – 4 mm, radially moderate deep to deep sulcate, crateriform; aerial mycelium absent; reverse black. Colony diam (in mm after 21 d) at 30 °C/32 °C: MEA 7– 9/no growth (ng) to 4, CYA 5 – 6/ng, PDA 6 – 8/ng to 2, OA 8 –10/ng to 2. No growth on MEA, CYA, PDA and OA at 35 °C.

Typus. USA, Oklahoma, McAlester, East side of building, outside wall, alcohol distillery, swab, 20 Jan. 2016, isol. Ž. Jurjević (holotype BPI 911212, culture ex-type CCF 5751 = CBS 146146 = EMSL 3307; ITS, LSU, SSU and β-tubulin sequences GenBank LR736040, LR736041, LR736042 and LR736049).

Additional materials examined. USA, Oklahoma, McAlester, East side of building, outside wall, alcohol distillery, swab, 20 Jan. 2016, Ž. Jurjević (culture CCF 5712 = CBS 142814 = EMSL 3308; ITS, LSU and SSU sequences GenBank LR736043, LR736044 and LR736045); South Carolina, outside wall, alcohol distillery, Oct. 2017, Ž. Jurjević (culture EMSL 4482; ITS sequence GenBank LR736046); ibid., (culture EMSL 4484; ITS sequence GenBank LR736047); ibid., (culture EMSL 4485; ITS sequence GenBank LR736048).

Notes — BLAST analysis with the ITS sequences of M. oklahomaensis showed low similarity with members of different genera, including Austroafricana parva (91.5 – 92 %), Pseudotaeniolina globosa (91.7 %) and Camarosporula persooniae (91.1 %), other taxa had similarity lower than 91 %. The LSU nrDNA sequence showed 94– 95 % similarity to a wide variety of genera in the Teratosphaeriaceae with Devriesia shelburniensis having the highest degree of similarity (94.9 %). The position of Muriphila within Teratosphaeriaceae is unresolved. Neither LSU nor SSU phylogenetic analyses comprising Teratosphaeriaceae genera (Quaedvlieg et al. 2014) were able to resolve its position with satisfactory support. In the resulting phylogenetic trees, the genus Muriphila was most commonly placed close to genera Batcheloromyces and Devriesia (data not shown).