Heracleicola premilcurensis Tibpromma, Camporesi & K.D. Hyde.
Index Fungorum number: IF551381; Facesoffungi number: FoF00921; Fig. 1
Etymology – refers to the name of the municipality in Italy where the species was collected.
Holotype – MFLU 14-0725
Saprobic on decaying plant stem of Heracleum sphondylium. Sexual morph: Ascomata 211 – 272 μm high × 150 – 221 μm diam. (x̄ = 254 × 196 μm, n = 5), immersed, visible as shiny, raised dots on the host surface, vaselike, solitary or scattered, with central short papilla, dark brown to black. Peridium 37 – 43 μm wide, a single stratum, comprising relatively large (6 – 9 μm), thickwalled, dark brown cells of textura angularis to t. globulosa. Hamathecium comprising numerous, 0.7 – 1.3 μm wide, long, filiform, frequently anastomosing, cellular, pseudoparaphyses. Asci 50 – 94 × 11 – 16 μm (x̄ = 72 × 14 μm, n = 10), 8 – spored, bitunicate, fissitunicate, cylindric-clavate, short pedicellate, rounded at the apex, with a wide, shallow, ocular chamber. Ascospores 23 – 36 × 6 – 9 μm (x̄ = 27 × 7 μm, n = 15), overlapping 1 – 2 – seriate, hyaline, 3 – septate, fusoid with rounded ends, cell above central septum often enlarged, constricted at the septum, guttulate, smooth – walled, lacking a mucilaginous sheath. Asexual morph: undetermined.
Culture characteristics – on MEA reaching 4 cm diam. after 1 week at 16 °C, later with dense mycelium, with irregular, rough margin, flattened, brown to black; hyphae septate, branched, light-brown, thick – walled.
Material examined – ITALY, Premilcuore, Province of Forlì-Cesena,, Valbura, on dead stem of Heracleum sphondylium (Apiaceae), 6 June 2014, Erio Camporesi IT1916 (MFLU 14-0725, holotype, HKAS, isotype); extype living culture, MFLUCC 14-0518, KUN; Ibid. (MFLU 15-1475, HKAS, isotypes); (MFLU 15-1476 bis, MFLU 15- 1477 tris, paratypes).
Fig. 1 Heracleicola premilcurensis (holotype) a, b Appearance of fungus on host surface c Cross section of ascoma d Ostiole e Section of peridium f Pseudoparaphyses g – i Asci j – l Ascospores m Germinating ascospore. Scale bars: a = 400 μm, b = 100 μm, c = 50 μm, d – e = 20 μm, f = 2 μm, g – i = 20 μm, j – l = 10 μm, m = 20 μm.