Dothiorella ostryae Manawasinghe, Camporesi & K.D. Hyde, sp. nov.

MycoBank number: MB 556874; Index Fungorum number: IF 556874, Facesoffungi number: FoF 06578; Fig. 16

Etymology: Name reflects the host genus of Ostrya carpinifolia.

Holotype: MFLU 18-0177.

Saprobic on dead aerial branch of Ostrya carpinifolia. Sexual morph Undetermined. Asexual morph Conidiomata pycnidial, stromatic, solitary, dark brown, semiimmersed, unilocular, globose papillate with a short neck, wall 5–7 cell layers, outer layers composed of light-brown cells of textura angularis, inner layer hyaline and thin-walled Conidiophores reduced to conidiogenous cells. Conidiogenous cells holoblastic, hyaline, cylindrical, proliferating percurrently. Conidia 15–21 × 7–10 ( = 19 × 9 µm, n = 30) μm, ellipsoidal initially hyaline and aseptate, becoming pale brown while attached to the conidiogenous cells, finally dark brown or sepia, 1-septate, with 2 cells of equal length, thick-walled.

Culture characteristics: Colonies on PDA reach 70 mm after 5 d in the dark at 25 °C.

Material examined: Italy, Passo delle Forche – Galeata (province of Forlì-Cesena), dead aerial branch of Ostrya carpinifolia L. (Betulaceae), 9 April 2018, Erio IT Camporesi (MFLU 18-0177, holotype), ex-type living culture, JZB3150026.

GenBank number: ITS: MN533805, tef1 MN537429.

Notes: The species identified in this study is similar to other species in Dothiorella (Phillips et al. 2013). In phylogenetic analysis (Fig. 14), Dothiorella ostryae is closely related to the clade that contains Dothiorella citricola (ICMP 16828) and D. viticola (CBS 117009). There are four Dothiorella species associated with the host genus Ostrya, namely D. iberica, D. omnivora, D. parva (Farr and Ross- man) and D. ostryae (this study).

Fig. 14 Phylogram generated from maximum likelihood analysis (RAxML) of Botryosphaeriales based on ITS and LSU sequence data. Maximum likelihood bootstrap values equal to or greater than 70%, Bayesian posterior probabilities equal to or greater than 0.90 (MLBS/PP) are given at the nodes. Isolate numbers are noted after each species name. The tree is rooted to Patellaria quercus (CPC 27232 and BHI F828a). Newly sequence data generated in this study are in blue. Ex-type and references strains are indicated in bold. Hyphen (-) represents support values less than 70% MLBS and 0.90 PP

Fig. 16 Dothiorella ostryae (MFLU 18-0177, holotype). a Material examined. b Submerged conidiomata on host. c, d Cross section of conidiomata on host. e Developing conidia while attached to the pyc- nidial wall on host. f, g Conidia on host. h Conidiogenous cells in culture. 1 Upper view of colony on PDA. j reverse view of 7 days old culture on PDA. Scale bars: a, b = 2 mm, c–h = 10 µm