Camarosporidiella laburnicola (R.H. Perera, Bulgakov & K.D. Hyde) Wanas. & K.D. Hyde, in Wanasinghe et al., Studies in Mycology 87, 234 (2017)

Index Fungorum number: IF 821953; Facesoffungi number: FoF 2784; Fig. 1

Saprobic on branches of Laburnum anagyroides. Sexual morph: Undetermined. Asexual morph: Conidiomata 300−350 µm diam., 210−300 µm high, pycnidial, solitary, scattered or grouped, semi-immersed to erumpent, globose, carbonaceous, black, with a papillate ostiole. Conidiomatal wall not observed. Conidiophores reduced to conidiogenous cells. Conidiogenous cells enteroblastic, with percurrent annellations, doliiform, hyaline, smooth-walled, formed from the innermost layer of the conidiomatal wall. Conidia 13−15 × 5−7 µm (x̄ = 14.1 × 6.4 µm, n = 20), oblong, ellipsoidal, straight to slightly curved, at first hyaline turning pale to dark brown at maturity, smooth-walled, rounded at both ends, muriform, 1–3-transversely septate and 1–2 vertical septa.

Culture characteristics – Colonies on MEA reaching 35–45 mm diam. in 4 weeks, powdery white to pinkish grey, spreading with moderate aerial mycelium, slightly irregular, margins smooth, with underneath pinkish grey.

Material examined – Italy, Lago Pontini – Bagno di Romagna, Forlì-Cesena, dead branches of Laburnum anagyroides, 3 November 2018, E. Camporesi, (MFLU 18-2630); living culture MFLUCC 22-0093).

GenBank numbers – LSU: OP680988, ITS: OP680989.

Known distribution (based on molecular data) – Italy (this study), Russia (Tibpromma et al. 2017).

Known hosts (based on molecular data) Laburnum anagyroides (Tibpromma et al. 2017, this study).

Notes – The sexual morph of Camarosporidiella laburnicola was introduced from Laburnum anagyroides in Russia (Tibpromma et al. 2017). This study recovered an isolate (MFLUCC 22-0093) from branches of L. anagyroides in Italy. This new isolate shares a close phylogenetic affinity to C. laburnicola (MFLUCC 14-0565) in our combined LSU and ITS sequence analyses. Our species is characterized by an asexual morph (Fig. 1) and cannot be compared with C. laburnicola (MFLUCC 14-0565), which has a sexual morph. A pairwise alignment of the ITS sequence of C. laburnicola (MFLU 15-1522) to that of our strain (MFLUCC 22-0093) revealed zero base pair differences. Therefore, we provide the first asexual morph report of C. laburnicola and a new geographical record for Italy.

Fig. 1  Camarosporidiella laburnicola (MFLU 18-2630, a new geographical record). a, b Conidiomata on host surface. c–h Conidiogenous cells and developing conidia. i–s Conidia. Scale bars: a = 2000 µm, b = 1000 µm, c–s = 10 µm.

Fig. 2 Phylogram generated from maximum likelihood analysis based on combined LSU and ITS sequence data. One hundred and eighteen strains are included in the combined gene analyses comprising 1327 characters after alignment (850 characters for LSU and 477 characters for ITS). Staurosphaeria lycii isolates (MFLUCC 17-0211, MFLUCC 17-0210) are used as the outgroup taxa. The tree topology of the Bayesian analysis was similar to the maximum likelihood analysis. The best RaxML tree with a final likelihood value of -2861.045329 is presented. The matrix had 168 distinct alignment patterns, with 2.83% undetermined characters or gaps. Evolutionary models GTR+I+G and GTR+G were selected as the best-fit models for the LSU and ITS gene regions, respectively. Bootstrap values for maximum likelihood equal to or greater than 50 and Bayesian posterior probabilities equal to or greater than 0.90 are placed above or below the branches, respectively. Ex-type strains are in bold. The newly generated sequences are indicated in yellow.

Fig. 2 Continued.