Botryosphaeria dothidea (Moug.) Ces. & De Not., Comm. Soc. crittog. Ital. 1(fasc. 4): 212 (1863)
≡ Sphaeria dothidea Moug., in Fries, Syst. Mycol. 2: 423 (1823)
= Botryosphaeria berengeriana De Not., Sfer. Ital. 82 (1863) 
= Fusicoccum aesculin Corda in Sturm, Deutschl. Fl., Abth. 3, 2:111 (1829)
Index Fungorum number: 183247, Faces of fungi number: FoF 03512
Saprobic on submerged wood of freshwater. Sexual morph Ascomata 300–500 μm diam., black, circular or subglobose to globose, scattered, gregarious, uni- to multi-loculate, immersed to erumpent on host tissue, with visible black dots or papilla. Ostiole circular, central, papillate. Peridium composed of two-layered locules, outer layer composed of dark brown or brown thick-walled cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis lining the locule. Pseudoparaphyses 2–4 μm wide, hyphae-like, septate. Asci 96–144 × 18–25 μm ( = 116 × 23 μm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, short pedicellatea, pically rounded with an ocular chamber. Ascospores 21–32 × 10–14 μm ( = 26.3 × 11.7 μm, n = 40), biseriate, hyaline, aseptate, fusoid to ovoid, sometimes with tapered ends, spindle-shaped, thin-walled, smooth with granular contents. Asexual morph Undetermined.
Cultural characteristics: Ascospore germinating on PDA within 24 hours. Colonies on PDA fast-growing, reaching 7–8 cm diameter in 50 days at 20–25°C, with dense, hairy, black mycelium on the surface, reverse black.
Material examined: China, Yunnan Province, small river of Puzhehei wetland, on dead submerged decaying wood of unidentified plants, 23 June 2019, Hao Yang, p27 (IFRD500–008), living culture = KUMCC 20–0186.
Known hosts: Olea europaea, Populus nigra, Prunus sp., submerged decaying wood, Vitis vinifera
Distribution: China, Greece, Portugal, Switzerland
Notes: Botryosphaeria dothidea is the type species of the genus, and was reported extensively from all around the world (Fries 1823, Arx and Müller 1954). It was epitypified by Slippers et al. (2004) based on morphology and phylogeny An isolate of this taxon was collected from southwest of China and its ITS sequence data of our isolate are 100% identical to the verified sequences of B. dothidea (MH992666 and MH973592). Therefore, we identify the strain as B. dothidea. It is a new geographic record from China and a new habitat record from freshwater.
GenBank numbers: LSU: MT559116; ITS: MT559099
Fig. 1 Botryosphaeria dothidea. a, b Appearance of ascomata on host substrate. c Section of ascoma. d Peridium. e Pseudoparaphyses and asci. f–i Asci. j–l Ascospores. m–n Colony on PDA (m from front, n from reverse). Scale bars: a = 500 μm, b = 200 μm, c, e = 100 μm, d = 30 μm, f–i = 20 μm, j–l = 10 μm.