Triplosphaeria guizhouensis. sp. nov.

MycoBank number: MB; Index Fungorum number: IF; Facesoffungi number: FoF 10694;

Etymology: referring to the collecting site of a small stream.


Saprobic on decaying, submerged wood in freshwater habitats. Sexual morph: Undetermined. Asexual morph: Colonies on natural substrate effuse, consisting of individual conidia scattered over the substrate surface, conspicuous, brown to black. Mycelium mostly immersed, consisting of branched, septate, smooth, subhyaline to pale brown hyphae. Conidiophores absent. Conidiogenous cells monoblastic. Conidia solitary, straight or slightly curved, cheiroid, quadrangular, pale brown to brown, with 4 arms, 5–7-septate each arm, 18.9–23 × 8–9 μm ( = 21 × 8.5 μm, n = 15) (conidia body not included appendages), guttulate, smoothwalled or sometimes verrucose on the base cell; with 3–4 (mostly 4) setose appendages, appendages (4.7–) (5.3–) (7.8–) 12.6–18.9 (–21.5) × 1–1.5 μm ( = 14.7 × 1.2 μm, n = 20), 2–4-septate, smooth.

Cultural characteristics: Conidia germinating on WA and germ tubes produced from appendages within 12 h. Colonies on PDA growing slowly, reaching 20–25 mm DIAM. after one month at 25°C, under the condition of the dark, circular, consisting of a matted felt with velutinous appearance, umbonate, surface of the colony mouse grey, in reverse brown.

Material examined: Chishui river basin, the north of Guizhou Province, CHINA, near 28°25′ N, 106°0′ E, at an altitude of 204 m, saprobic on submerged decaying wood in freshwater stream, July 2019, Lingling Liu, CS1-18-1 (GZAAS20-0407, holotype), ex-type living culture GZCC19-0512.