Phylogenetic relationships inferred from neighbour-joining analysis of ITS-rDNA sequences of 22 taxa. The optimal tree with the sum of branch length = 0.82684195 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The novel Indian species: Austroboletus appendiculatus having GenBank numbers accession number KX530028 (ITS-rDNA) is shown in bold and blue. The topology is rooted with Boletus edulis. Evolutionary analysis was conducted in MEGA 6.0 (Tamura 2013).