Walkaminomyces medusae – Facesoffungi number: FoF 11227

Walkaminomyces medusae (Carnegie & G.S. Pegg) Crous & Carnegie, comb. nov.

MycoBank number: MB 832057; Index Fungorum number: IF 832057; Facesoffungi number: FoF 11227;

Basionym: Mycosphaerella medusae Carnegie & G.S. Pegg, Australas. Pl. Path. 40: 376. 2011.

Diagnosis: Leaf spots amphigenous, irregular, small (5 mm diam) to large spreading blights with irregular margins, greyish brown to reddish brown with a prominent purple to purplish green diffuse border. Ascospores multiseriate, overlapping, hyaline, guttulate, straight, fusoid-ellipsoidal with obtuse ends, obviously widest in middle of apical cell, medianly 1-septate, not constricted at septum or slightly so, tapered to both ends but more prominently to apical end, 10– 12 × 3– 4 μm.

Description and illustration: Carnegie et al. (2011).

Typus: Australia, Queensland, Walkamin, on E. alba, 11 Nov. 2008, A.J. Carnegie, M. Ramsden & G.S. Pegg (holotype BRIP 52586, culture ex-type BRIP 52586 = CBS 130521).

Notes: Based on a megablast search of NCBI’s GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Hyalozasmidium aerohyalinosporum [GenBank NR_156220.1; Identities = 497/514 (97 %), 7 gaps (1 %)], Hyalozasmidium sideroxyli [GenBank NR_156372.1; Identities = 423/446 (95 %), 5 gaps (1 %)], and Madagascaromyces intermedius [GenBank MH863356.1; Identities = 483/516 (94 %), 10 gaps (1 %)]. Closest hits using the LSU sequence are Paramycosphaerella marksii [GenBank DQ204758.1; Identities = 815/822 (99 %), 1 gap (0 %)], Paramycosphaerella intermedia [GenBank NG_059428.1; Identities = 813/822 (99 %), 1 gap (0 %)], and Hyalozasmidium aerohyalinosporum [GenBank NG_059440.1; Identities = 812/ 822 (99 %), 1 gap (0 %)]. Closest hits using the rpb2 sequence had highest similarity to Hyalozasmidium sideroxyli [GenBank MF951505.1; Identities = 668/805 (83 %), 10 gaps (1 %)], Paramycosphaerella marksii [GenBank MF951573.1; Identities = 624/774 (81 %), 4 gaps (0 %)], and Paramycosphaerella intermedia [GenBank MF951568.1; Identities = 623/774 (80 %), 4 gaps (0 %)].