Trichoderma pinicola S-Y. Oh, M.S. Park & Y.W. Lim
Index Fungorum number: IF 824662; Facesoffungi number: FoF 04460
Etymology: The specific epithet ‘‘pinicola’’ refers to the genus name of Pinus densiflora, the source of the type strain.
Colour codes follow: Methuen Handbook of colour (Kornerup and Wanscher 1963).
Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Conidiophores branched at an acute to the main axis, mostly paired. Phialides 7.8–13.3 × (2.5-)2.6–4.1(-4.5) μm, l/w 1.9–4.4(-4.5) × 1.8–3.3 μm wide at the base (n = 30), typically formed in whorls of 3 or rarely solitary, subulate or lageniform. Conidia 3.5–4.9(-5) × 2.8–3.5(-3.6) µm, l/w 1.1–1.6 (n = 30), smooth, subglobose to ellipsoid. Chlamydospores rare, globose, 5.2–10(-10.1) × 5.2–10 μm, l/w 1–1.02 (n = 10). No distinct odour. Agar not pigmented.
Culture characteristics: On CMD after 72 h, colony radius 14–18 mm at 15 °C, 23–34 mm at 20 °C, 39–50 mm at 25 °C, 34–45 mm at 30 °C; colonies on CMD fill a 90 mm diam. Petri dish at 4 days at 25 °C. Colony hyaline, radial, not zonate, mycelium common, aerial hyphae inconspicuous. Conidiation starting after 4 days, greyish green (M. 28B5) or deep green (M. 28E8) conidia formed scarcely on aerial hyphae around the margin of the colony. On PDA after 72 h, colony radius 12–15 mm at 15 °C, 18–28 mm at 20 °C, 30–38 mm at 25 °C, 21–36 mm at 30 °C; colonies on PDA fill a 90 mm diam. Petri dish within 6 days at 25 °C. Colony hyaline, radial, with a wavy margin, mycelium common, aerial hyphae more abundant in the colony centre. Conidiation starting after 9 days, greyish green (M. 27C6) or pastel green (M. 29A4) conidia formed on aerial hyphae, more abundant around the margin of the colony. On SNA after 72 h, colony radius 9–12 mm at 15 °C, 19–24 mm at 20 °C, 30–43 mm at 25 °C, 19–33 mm at 30 °C; colonies on SNA fill a 90 mm diam. Petri dish within 5–6 days at 25 °C. Colony hyaline, radial, and mycelium loose, spreading uniformly throughout the colony. Conidiation starting after 3–4 days, greyish green (M. 29C5) or deep green (M.27E8) conidia formed abundant on aerial hyphae.
Material examined: REPUBLIC OF KOREA, Gangwon Province, Hongcheon, Gongjaksan Mountain, elev. 300 m, isolated from the root of Pinus densiflora under a fairy ring of Tricholoma matsutake, September 2013, S-Y. Oh, PF233 (SFC20130926-S233, holotype), ex-type living culture KACC 48486; ibid., Hongcheon, Gongjaksan Mountain, elev. 300 m, isolated from the root of P. densiflora under a fairy ring of T. matsutake, September 2013, S-Y. Oh, SFC20130926-S111.
GenBank numbers: ITS = MH050354, RPB2 = MH025993, TEF1-a = MH025981.
Notes: Trichoderma pinicola is morphologically similar to T. hirsutum K. Chen & W.Y. Zhuang, the latter has longer phialides (11.4–18.3 × 2.5–3.1 lm) and lacks chlamydospores in CMD. Phylogenetically, T. pinicola is closely related to T. simplex K. Chen & W.Y. Zhuang. However, T. simplex has more abundant aerial hyphae and conidia formation and faster growth at 25 °C (49–56 mm on CMD and 52–56 mm on PDA after 3 days). In addition, T. pinicola showed a wavy margin on PDA.
Fig. 1 Trichoderma pinicola (SFC20130926-S233, holotype). a Colony on CMD after 72 h. b Colony on PDA after 72 h. c Colony on SNA after 72 h. d–f Conidiophores. g Chlamydospores. h Conidia. Scale bars d–h = 20 μm.