Pseudocyclothyriella clematidis (Phukhams. and K. D. Hyde) Phukhams. and Phookamsak, comb. nov., Figure 3

Index Fungorum Number: IF 557442 Facesoffungi Number: FoF 09540

Pseudocoleophoma clematidis Phukhams. and K. D. Hyde, in Phukhamsakda et al., Fungal Divers (102): 21 (2020).

Holotype Details: Italy, Arezzo Province, Badia Tega— Ortignano Raggiolo, on dead aerial branch of Clematis vitalba,

March 9, 2013, E. Camporesi, IT 1110 (MFLU 16-0280), ex-type living culture, MFLUCC 17-2177.

Description and Illustration: See Phukhamsakda et al. (2020).

Ecology and Known Distribution: As a saprobe, Pseudocyclothyriella clematidis has contributions in the cycle of the material. To date, the species is just reported in Italy (Phukhamsakda et al., 2020).

Notes: Pseudocyclothyriella clematidis was previously treated in Pseudocoleophoma based on   phylogenetic evidence (Phukhamsakda et al., 2020). Pseudocyclothyriella clematidis differs from the other Pseudocoleophoma species in having yellowish brown, oval to oblong, aseptate conidia (Phukhamsakda et al., 2020). A morphological comparison of P. clematidis with other Pseudocoleophoma species is provided in Table 3. The two strains of Pseudocyclothyriella clematidis (MFLU 16-0280 and MFLUCC 17-2177A) formed a strongly supported clade in this study. Phukhamsakda et al. (2020) introduced Pseudocoleophoma clematidis based on phylogenetic evidence and reported the species was poorly supported in a subclade in between the main clade of Pseudocoleophoma and P. typhicola (MFLUCC 16-0123). However, in our multigene phylogeny, we recovered the same taxon as basal to Pseudoconiothyrium broussonetiae.

TABLE 3 | Synopsis of morphological characteristics of Pseudocoleophoma and Pseudocyclothyriella.

Species name Sexual morph Asexual morph References
Ascomata Asci Ascospores Conidiomata Conidiogenous cells Conidia
Pseudocoleophoma bauhiniae 100–

120 × 125–

145 µm, solitary or scattered, dark

65–80 × 5–8 µm,

clavate to cylindric-clavate

17–20 × 3.5–

4.5 µm, hyaline, cylindric- fusiform,

130–150 × 90–

115 µm, immersed to superficial, globose to subglobose,

2.5–5.5 × 2–3 µm,

phialidic, hyaline, doliiform to lageniform

7.5–11 × 2–

3 µm, hyaline, oblong to ellipsoidal, with rounded or

Jayasiri et al., 2019
P. calamagrostidis brown,

subglobose to obpyriform, coriaceous

160–

220 × 140–

200 µm, scattered, dark

62.5–80 × 7.5–

10 µm, cylindrical

1–3-septate,

without sheath

16–19 × 3–

4.5 µm, hyaline, narrowly

glabrous,

multiloculate, ostiolate

250–500 × 220–

300 µm, immersed to erumpent, depressed globose,

5–9 × 2–4 µm,

phialidic, hyaline, doliiform to subglobose

obtuse ends,

aseptate

6–10 × 2–

2.5 µm, hyaline, cylindrical,

Tanaka et al., 2015
P. flavescens brown, globose

to depressed globose

N/A

N/A fusiform,

1-septate, with sheath

N/A

glabrous,

uniloculate, ostiolate

20–140 µm diam., solitary or confluent, globose, glabrous or covered by hyphae

4–6 × 3–6 µm,

globose to doliiform

aseptate

4–7 × 2–

3.5 µm, hyaline, oblong to ellipsoidal

with two very

De Gruyter

et al., 1993; Li et al., 2020

P. polygonicola 280–

350 × 230–

310 µm, scattered to

2–4-gregarious,

74–90 × 9–

12.5 µm, cylindrical to clavate

19–23 × 4–

6 µm, hyaline, fusiform,

1-septate, with

sheath

170–250 µm

diam., superficial, ampulliform, glabrous, uniloculate,

7–17 × 3.5–5 µm,

phialidic, hyaline, doliiform to lageniform

large polar guttules, aseptate

11.5–18 × 3–

4.5 µm, hyaline cylindrical, aseptate

Tanaka et al., 2015
P. rusci brown to dark brown, globose to subglobose

N/A

N/A N/A ostiolate

130–200 × 250–

330 µm, deeply immersed, globose, subglobose or

4–9 × 3–7 µm,

enteroblastic, phialidic, hyaline, doliiform,

8–14 × 3–

6 µm, hyaline,

cylindrical to subcylindrical

Li et al., 2020
P. typhicola N/A N/A N/A ovoid, glabrous,

uniloculate, ostiolate

60–100 × 140–

150 µm,

semi-erumpent, subglobose,

ampulliform to

subcylindrical

2–5 × 2–5 µm,

enteroblastic,

hyaline, subcylindrical

or fusiform,

aseptate

9–11 × 2–

3 µm, hyaline,

oblong to cylindrical,

Hyde et al., 2016
P. zingiberacearum N/A N/A N/A glabrous, uniloculate

200–220 × 110–

150 µm, immersed,

depressed globose, glabrous,

1.5–2.5 × 1–

1.5 µm, phialidic,

hyaline, doliiform to lageniform

1-euseptate

12–14 × 2–

3 µm, hyaline,

oblong to ellipsoidal,

Tennakoon et al., 2019
Pseudocyclothyriella

clematidis (≡ Pseudocoleophoma clematidis)

 

N/A N/A N/A multiloculate, non-ostiolate 130–150 × 100–130 µm, immersed, globose to subglobose, glabrous, uniloculate, ostiolate 2–4 × 1.5–4 µm, holoblastic, phialidic, hyaline, cylindrical to subcylindrical aseptate

5–8 × 2–4 µm, yellowish brown, oval, aseptate

Phukhamsakda et al., 2020; This study

FIGURE 3 | Pseudocyclothyriella clematidis (MFLU 16-0280, holotype). (a,b) Appearance of pycnidial conidiomata on host substrates. (c) Vertical section of conidioma. (d) Ostiole. (e) Pycnidial wall. (f,g) Conidiogenous cells with attached conidia. (h) Conidia. (i) Culture colony on MEA (upper view). Scale bars: (b) = 500 µm, (c) = 200 µm, (d,e) = 20 µm, and (f–h) = 5 µm.

Species