Pestalotiopsis cyclobalanopsidis N. Jiang, in Jiang, Voglmayr, Xue, Piao & Li, Microbiology Spectrum: 10.1128/spectrum.03272-22, 11 (2022)
Index Fungorum number: IF 841310; MycoBank number: MB 841310; Facesoffungi number: FoF 11693;
Etymology – named after the host genus, Cyclobalanopsis.
Pathogenic to host leaves. Asexual state: Conidiomata in culture sporodochial, solitary, erumpent, pulvinate, dark brown, 250–700 μm diam., exuding black conidial masses. Conidiophores indistinct, usually reduced to conidiogenous cells. Conidiogenous cells hyaline, smooth, cylindrical to sphaerical, annelidic, 6–8 × 2–4.5 μm, mean ± SD = 7.2 ± 0.9 × 3.6 ± 0.8 μm. Conidia fusoid, curved, 4-septate, smooth, slightly constricted at the septa, (18.5–)20–24.5(–25.5) × (6–)6.5–8(–8.5) μm, mean ± SD = 22.3 ± 1.3 × 7.3 ± 0.5 μm (n = 50), L/W = 2.6–3.6; basal cell obconic with a truncate base, thin-walled, hyaline to pale brown, 4–6(–6.5) μm; median cells 3, trapezoid or subcylindrical, concolourous, brown, thick-walled, the first median cell from base 4.5–6(–6.5) μm long, the second cell (3.5–)4–5 μm long, the third cell (4–)4.5–5.5 μm long, together 13–15.5(–16) μm long; apical cell conic with an acute apex, thin-walled, hyaline to pale brown, 3–4 μm long; basal appendage unbranched, tubular, centric, straight, (2–)2.5–5.5(–6.5) μm long, mean ± SD = 3.8 ± 1.5 μm; apical appendages 2–5, unbranched, tubular, bent, (5.5–)7–13(–14.5) μm long, mean ± SD = 10.1 ± 3 μm. Sexual morph unknown.
Culture characters – Colonies on MEA flat, spreading, with flocculent aerial mycelium forming concentric rings and entire, hazel to off-white, reaching a 70-mm diameter after 10 days at 25°C, sterile; on PDA, flat, spreading, with flocculent aerial mycelium forming concentric rings and undulate edge, white to luteous, reaching a 50-mm diameter after 10 days at 25°C, forming dark brown conidiomata with black conidial masses.
Material examined – China, Guangdong Province, Shaoguan City, Lechang City, Dayaoshan Forest Farm, on diseased leaves of Cyclobalanopsis glauca (Fagaceae), 4 December 2019, Shang Sun (holotype CAF 800022 = JNH0018; ex-holotype culture CFCC 54328); ibid., CFCC 55891.
Distribution – China
Sequence data – ITS: OK339735 (ITS1/ITS4); tef1: OK358496 (EF1-728F/EF2); tub2: OK358511 (Bt2a/Bt2b)
Notes –Pestalotiopsis cyclobalanopsidis is closely related to P. castanopsidis, P. guizhouensis, and P. jesteri (Fig. 1). However, P. cyclobalanopsidis differs from them by distinctly curved conidia (24).
Fig. 10. Morphology of Pestalotiopsis cyclobalanopsidis (CFCC 54328). A. Colony on PDA after 10 d at 25 °C; B. Colony on MEA after 10 d at 25 °C; C. Conidioma formed on PDA; D–F. conidiogenous cells giving rise to conidia; G. conidia. — Scale bars: C = 500 μm; D–G = 10 μm.