Nothoramichloridium perseae Crous, in Crous et al., Persoonia 43: 279 (2019)

MycoBank number: MB 832878; Index Fungorum number: IF 832878; Facesoffungi number: FoF 14953;

Etymology. Name refers to the host genus Persea from which it was isolated.

Classification — Phyllostictaceae, Botryosphaeriales, Dothideomycetes.

Mycelium consisting of hyaline, smooth, septate, branched, 1.5 – 2.5 µm diam hyphae. Conidiophores solitary, erect, flexuous, subcylindrical, brown, finely verruculose, 2 – 3-septate, 80 –150 × 4 – 5 µm. Conidiogenous cells terminal, integrated, subcylindrical, straight to geniculous-sinuous, 40 –70 × 4–5 µm; upper part forming a rachis with tightly aggregated sympodial loci, truncate, subdenticulate, 1 µm diam, not thickened nor darkened. Conidia solitary, clavate, pale brown, guttulate, thick-walled, verruculose, straight, apex obtuse, base truncate, 1 µm diam, not thickened nor darkened, 1(– 2)-septate, with septa forming a protruding rift visible in conidial outline, (19 –)21– 23(– 26) × 5(– 6) µm.

Culture characteristics — Colonies erumpent, spreading, with moderate aerial mycelium and smooth, lobate margin, reaching 10 mm diam after 2 wk at 25 °C. On MEA surface rosy buff, reverse rosy buff to isabelline. On PDA surface buff to isabelline, reverse isabelline. On OA surface buff.

Typus. SOUTH AFRICA, Mpumalanga Province, Nelspruit, on leaves of Persea americana (Lauraceae), 9 Aug. 2014, P.W. Crous, HPC 2565 (holotype CBS H-24245, culture ex-type CPC 36383 = CBS 146048.

GenBank accession numbers — ITS: MN562121.1, LSU: MN567629.1.

Notes — Nothoramichloridium is phylogenetically allied to Anungitiomyces and Strelitziomyces, and these genera represent an undescribed family in the Xylariales (Crous et al. 2019a).

Based on a megablast search of NCBIs GenBank nucleotide database, the closest hits using the ITS sequence had highest similarity to Hypoxylon lenormandii (voucher EBS228, GenBank KM610287.1; Identities = 330/377 (88 %), 18 gaps (4 %)), Rhinocladiella pyriformis (strain CBS 469.94, GenBank MH862476.1; Identities = 393/449 (88 %), 14 gaps (3 %)), and Anungitiomyces stellenboschiensis (strain CPC 34726, GenBank MK876376.1; Identities = 374/428 (87 %), 15 gaps (3 %)). Closest hits using the LSU sequence are Anungitiomyces stellenboschiensis (strain CPC 34726, GenBank MK876415.1; Identities = 828/841 (98 %), 1 gap (0 %)), Oxydothis garethjonesii (strain MFLUCC 15-0287, GenBank KY206762.1; Identities = 827/863 (96 %), 4 gaps (0 %)), and Arthrinium malaysianum (strain CBS 102053, GenBank NG_042780.1; Identities = 826/864 (96 %), 4 gaps (0 %)).