Mangifericomes hongheensis E.F. Yang & S. Tibpromma, sp. nov.

MycoBank number: MB 559284; Index Fungorum number: IF 559284; Facesoffungi number: FoF 10596;

Saprobic on decayed branch of Mangifera indica. Sexual morph: Ascomata 135–250 μm (x̅ = 188 μm, n = 20) high, 243–326 μm (x̅ = 285 μm, n = 20) diam., scattered to gregarious, globose to subglobose, semi-immersed to fully immersed, emerging as dark brown to black raised regions on host surface, ostiole. Peridium 20–35 µm wide, thick-walled, dark brown to black, multilayer, the upper part in contact with the bark, arranged in a textura angularis to globosa, 10–18 µm wide at the middle part. Hamathecium 1.5–2.5 μm wide, hyaline, septate, branched, cellular, pseudoparaphyses, embeded in a glutinous matrix. Asci 140–180 × 20–30 μm (x̅ = 165 × 25 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, with long or minute pedicel, apically rounded with a ocular chamber. Ascospores 30–40 × 12–16 μm (x̅ = 35 × 15 μm, n = 20), overlapping uni or biseriate, pale-brown to brown, ellipsoid, slightly bent to straight, muriform, rough-walled, slightly wider at near to apex, 7–11-transversally septate, and 5–8 longitudinal septum, slightly constricted at the septa, enclosed in a gelatinous sheath 8–11 µm wide. Asexual morph: Undetermined.

Culture characteristics: Ascospores germinated within 20 hours on WA, colonies on PDA reaching 7–12 mm diam. after 1 week at the PDA medium, circular, convex, raised, erose or dentate, moderately dense, above cottony with abundant white, slight gray to white from margin to center; reverse: visible white loop at margin, sunk at the center, brown to dark at center, without pigments produced in PDA.

Material examined: CHINA, Yunnan Province, Honghe Menglong village on dead branch of Mangifera indica, (102 ° 50’11”E, 23°41’01’’N, 500.6 m), 24 July 2019, E.F. Yang, EFH000 (KUN-HKAS 1221888), (living culture KUMCC 21-0342, holptype), ex-type culture KUMCC 21-0345

Note: Mangifericomes hongheensis KUMCC 21-0342 was identified as erritorial saprobic fungi associated with dead branch of Mangifera indica. The BLASTn (LSU, tef1–α and rpb2) and multi-genetic comparison excepted M. hongheensis KUMCC 21-0342 quite different in base pairs with other genera, Such as the top tef1–αrpb2 blastn results indicated 77% similarity with Bambusicolaceae spp. and Didymellaceae spp. Moverover the multi-gene analysis shown M. hongheensis well separated with other genera/families with high support bootstrap value (85% ML and 1.00 BI). Therefore, we introduced Mangifericomes as a new genus unique existence under order Pleosporales, with Mangifericomes hongheensis KUMCC 21-0342 as the type species.