Grigorovia Gouliamova, Dimitrov gen. nov.

Index Fungorum number: MB 820467; Facesoffungi number: FoF

Phylogenetic placement: Saccharomycetales, Saccharomycotina, Ascomy- cota.

Etymology: Gri-go-ro-via to honour a Bulgarian scientist Stamen Grig- orov who discovered Lactobacillus delbrueckii subsp. bulgaricus [17]. The new genus Grigorovia (Cluster 4) can be distinguished from the genera Kazachstania and Kluyveromyces by comparative analysis of combined LSU and ITS rDNA sequences (Fig. 1 [11]). Comparison of physiological profiles showed that char- acteristics with major contribution in average DPP between the new genus and Cluster 3 (Fig. 2 [11]) are as follows. Majority of yeast species of the genus Grig- orovia do not ferment sucrose and raffinose. All species of the genus Grigorovia do not assimilate sucrose. Majority of species of the genus Grigorovia (three out of four) assimilate L-lysine, while most species of Cluster 3 (ten out of twelve) do not assimilate it. Most species of the genus Grigorovia do not assimilate α, α- trehalose, while species of Cluster 3 assimilate it. Majority of species of the genus Grigorovia assimilate ethylamine and cadaverine, while most species of Cluster 3 do not assimilate them.

Sporulation. Budding cells are transformed directly into asci containing one to two, one to four globose to subglobose ascospores on McClary’s acetate, YM and corn meal agars.

Ecology and biogeography. Three species were isolated from soil and decaying leave samples collected in Southeast Asia (Taiwan and Japan). One species was isolated from soil in South Africa.

Fig.  1. Phylogenetic relationships  of  new  yeast  strains,  IMB190RΤ,  IMB190, IMBBR1,     IMBBR2       and members  of  the  Kazachstania  clade  inferred  using neighbour-joining analysis (Kimura 2 parameter model) of combined sequences of D1/D2 domains of the LSU rDNA and ITS regions. Kluyveromyces marxianus rep- resents the outgroup. Scale represents 0, 02 nucleotide substitutions per site

Fig. 2. Kazachstania chrysolinae IMB190RT (a) morphology of budding cells of new  yeast species in 5% glucose broth, scale – 5 µm, (b) morphology of ascospores of new yeast species in V8 juice agar