Flammeascoma bambusae Phookamsak & K.D.Hyde.
Index Fungorum number: IF550924, Facesoffungi number: FoF 00427, Fig. 1.
Etymology: The specific epithet bambusae refers to the host bamboo in reference to the host from which the fungus was isolated.
Holotypus: MFLU 11–0143
Saprobic on submerged bamboo. Sexual morph Ascostromata 400–630μm, high, 1000–1400 μm diam., solitary, gregarious, semi-immersed to superficial, dark at the basal region and orange brown at the apex of the ascoma, unito bi-loculate, conical to lenticular, with a flattened base, coriaceous, ostiole central, with pore-like opening. Peridium 70– 110 μm wide, thick-walled, of unequal thickness, poorly developed at the base, composed of several layers of brown to dark brown cells, with host cells plus fungal tissue, organised in a textura epidermoidea. Hamathecium comprising 1– 1.8μm wide, dense, narrow pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci (125–)130–160(– 175)×(14.5–)15–17(–20) μm (x=147.7×16.6μm, n=20), 8-
spored, bitunicate, fissitunicate, clavate to cylindric-clavate, short pedicellate with foot-like pedicel, apically rounded with well-developed ocular chamber. Ascospores (35–)40–43(–48)×7–9(–10.5) μm (x=41.9×8.7μm, n=25), overlapping 1–2-seriate, didymosporous, fusiform, slightly curved, hyaline, becoming brown when released from asci and geminating, 1-septate, constricted at the septa, swollen near the septa, with small and large guttules, wall rough, surrounded by mucilaginous sheath. Asexual morph Undetermined.
Culture characters: Colonies on PDA slow growing, 10–20 mm diam. after 4 weeks at 25–30 °C, dark-greenish to black at the margin, grey to dark grey in the centre; reverse dark-greyish to black; medium dense to dense, irregular, slightly raised to low convex or umbonate, dull with undulate edge, hairly to woolly, slightly wavy.
Material examined: THAILAND, Chiang Mai Province, Chom Tong District, Doi Inthanon, on dead stem of submerged bamboo (Bambusae), 5 September 2009, R.Phookamsak RP0013 (MFLU 11–0143, holotype); ex-type living culture, MFLUCC 10–0551. GenBank ITS: KP744440; SSU: KP753952.
Fig. 1 Flammeascoma bambusae (holotype) a Ascostromata visible as black raised structures, orangish at the apex, raised on host surface b Section through an ascoma c Section through peridium d Asci with narrow pseudoparaphyses e–g Asci h–l Ascospores m Spore germination. Scale bars: b=200μm, c=50μm, d–g, m=20μm, h–l=5μm.