Chrysoporthe cubensis (Bruner) Gryzenh. & M.J. Wingf., in Gryzenhout et al., Stud. Mycol. 50(1): 130 (2004).
Pathogenic on woody host forming cankers. Sexual morph: Ascostromata 120–230 μm high, 280–490 μm diam., semi-immersed in bark, recognizable by extending, fuscous-black, cylindrical perithecial necks, and in some cases, erumpent, limited, orange ascostromatic tissue. Ascostroma 170–250 μm diam., perithecial, valsoid, 1–9 perithecia per stroma, bases immersed in bark, black, globose. Peridium 17–22 μmthick, top of perithecial bases covered with cinnamon to orange, predominantly prosenchymatous, stromatic tissue forming a clypeus of variable extent, which is occasionally visible above the bark surface. Perithecial necks up to 240 μm long, 110–610 μm wide, black, periphysate, emerging through bark covered in umber stromatic tissue of textura porrecta, thus appearing fuscous-black, extending necks. Asci 25–34 × 4–6.5 μm, 8-spored, biseriate, unitunicate, free when mature, astipitate, with a non-amyloid refractive ring, fusoid to ellipsoid. Ascospores 6.5–7.5 × 2–2.5 μm, hyaline, one-septate, with septum variously placed in the spore but usually central, fusoid to oval, with tapered apices. Asexual morph: Conidiomata occurring separately or on the top of an ascostroma, distinguishable from ascomata by their pyriform shape, attenuated necks, conidiomatal locules and distinct stromatic tissue. Conidiomata 130–740 μm high, 100–950 μm diam., eustromatic, superficial to slightly immersed, pyriform to clavate, sometimes pulvinate, with one to four attenuated necks per structure, fuscous-black, with an umber interior when young, conidiomatal base above the bark surface. Necks up to 230 μm long, 90–240 μm wide. Conidiomatal locules 110–500 μm diam., with even to convoluted inner surface, occasionally multilocular, single locule connected to one or several necks. Stromatic tissue of base of textura globulosa with walls of outer cells thickened, and neck cells of textura porrecta. Conidiophores 4–7 × 3–5 μm, hyaline, with a globular to rectangular basal cell, branched irregularly at the base or above into cylindrical cells. Paraphyses or cylindrical cells 13–20 μm high, hyaline, septate or not. Conidiogenous cells 2–3 μm wide, phialidic, determinate, apical or lateral on branches beneath the septum, cylindrical to flask-shaped with attenuated apices, collarette and periclinal thickening inconspicuous. Conidia hyaline, nonseptate, oblong, 3.5–4.5 × 1.5–2.5 μm, exuded as bright luteous spore tendrils or droplets (description based on Gryzenhout et al. 2004).
Material examined: COLOMBIA, Darien, bark of Tibouchina semidecandra, 2001, R. Arbelaez, PREM 58022, holotype.
Notes: Chrysoporthe was introduced and typified by C. cubensis from Tibouchina. Asexual morph is prominent in this genus and most specimens only associated with asexual morph. We observed the holotype specimen of
Chrysoporthe cubensis and we could not see the sexual morph.
Fig. Chrysoporthe cubensis (PREM 58022). a Packet of herbarium. b Herbarium specimen. c Conidiomata on substrate. d Vertical cross section of conidiomata. e Stromatic tissues turn to purple in KOH. f, g Conidiogenous cells, conidia. h Conidia. Scale bars: c = 200 μm, d, e = 100 μm, f–h = 10 μm.