Aphanophora eugeniae (Crous & Alfenas) Réblová & Unter., PLoS ONE 8(5): e63547, 14 (2013)
= Cyphellophora eugeniae Crous & Alfenas, Persoonia 22: 147 (2009)
Index Fungorum number: IF803678
On PDA: Mycelium consisting of branched, greenish-brown, septate, smooth, 3–5 µm wide hyphae, constricted at septa. Conidiogenous cells phialidic, intercalary, inconspicuous to subdenticulate, 1 µm wide, with minute collarettes, with several loci aggregated at hyphal swellings. Conidia subcylindrical, tapering towards obtuse ends, curved, smooth, hyaline to olivaceous, finely guttulate, 4–6(–10)-septate, prominently constricted at septa, widest in the middle of conidium, (40–)60–75(–90) × 2–2.5(–3) µm; conidia also anastomose and undergo microcyclic conidiation in culture.
Characteristics in culture: Colonies on PDA erumpent, with sparse aerial mycelium and even margins; surface olivaceous-grey, with patches of iron-grey; reverse iron-grey. On MEA erumpent, with folded surface and smooth, lobate margin, and sparse aerial mycelium; surface pale olivaceous-grey to olivaceous-grey; reverse iron-grey. On OA spreading, flat, with even, smooth margins and sparse aerial mycelium, olivaceousgrey. Colonies reaching 15 mm diam after 1 mo at 25 °C, fertile, sporulating in slimy sporodochial masses.
Specimen examined: Brazil, Rio Grande do Sul, Guaiba, living leaves of Stenocalyx uniflorus, 1 Apr. 2008, leg. A.C. Alfenas, isol. P.W. Crous, holotype CBS H-20201, culture ex-type CPC 15172 = CBS 124105.
Figure 1. Aphanophora eugeniae. Living culture with detail of aerial mycelium (A, B), seta that occurs rarely in culture (C), phialides with multiple loci within inconspicuous collarettes (arrow indicates the collarette, D–H), and conidia undergoing microcyclic conidiation (I). DIC (C–F, H, I), PC (G), bar = 20 µm (C), 10 µm (A, B, D–I). CBS 124105 (ex-type).