Wiesneriomyces laurinus (Tassi) P.M. Kirk, Trans Br Mycol Soc 82: 748 (1984)
Index Fungorum number: IF 107371; MycoBank number: MB 107371; Facesoffungi number: FoF 09126, Fig. 1
Saprobic on dead leaves of Dimocarpus longan. Sexual morph: Not observed. Asexual morph: Colonies effuse, yellowish-brown. Conidiomata sporodochial, solitary, 3–10 setae arising from the margins of the sporodochial stalk. Setae 140–300 × 3–8 µm (x̄=180 × 5 μm, n=30), subulate, apex acute, septate, thick-walled, deep brown, arising on leaf surface. Conidiophores 30–50 × 2–4 µm (x̄=40×3 μm, n=21), emi-mucronematous, close to one another, brown to sub-hyaline at the base, hyaline towards the apex, septate, irregularly branched. Conidiogenous cells 6–9×2–4 µm (x̄=7×3 μm, n=26), located at the conidiophores terminal, cylindrical, hyaline, integrated. Conidia 50–70×2–4 μm (x̄=60×3 μm, n=30), solitary, clumped together into a semi-mucus, hyaline, smooth, sandy, cylindrical, taper towards both ends that are obtusely rounded, 5–6-septate, prominently constricted at septa, median cells 6–10×2–4 µm long, terminal cells 6–10×2–4 µm.
Culture characteristics – Colonies on PDA, 37–42 mm diam. after 3 weeks, colonies from above: medium dense, flat, slightly raised, rough surface with irregular edges, fluffy not smooth, leaden grey to light grey at the margin, black to ollvnceous black in the centre; reverse: grey to light grey at the margin, grey-brown to grey in the centre.
Material examined – China, Guangdong Province, Guangzhou City, Haizhu District, Zhongkai University of Agriculture Engineering, 23° 6′ 32″ N, 113° 16′ 37″ E, alt. 20 m, on the dead leaves of Dimocarpus longan Lour. (Sapindaceae), 23 July 2021, YH. Yang & CF. Liao (ZHKU 22-0008); living cultures ZHKUCC 22-0008, ZHKUCC 22-0009.
Known hosts – Carissa carandas (Apocynaceae), Clusia rosea (Clusiaceae), Hibiscus elatus (Malvaceae), Ficus ampelas (Moraceae), Gyranthera caribensis (Malvaceae), leaf litter of Caesalpinia echinata (Fabaceae), Laurus nobilis (Lauraceae), Ocotea leucoxylon (Lauraceae), Pandanus urophyllus (Pandanaceae), Psidium guajava (Myrtaceae), Roystonea regia (Arecaceae), Talipariti elatum, Theobroma cacao (Malvaceae), Thuja occidentalis (Cupressaceae), Dimocarpus longan (Sapindaceae)
Distribution – Brazil (da Silva and Grandi 2008), China (this study), Cuba (Delgado-Rodriguez et al. 2002), Hong Kong (Lu et al. 2000; Zhuang 2001), Mexico (Begerow et al. 2018), Myanmar (Thaung 2008), Russia (Melnik and Popushoi 1992), United Kingdom (Dennis 1986), Venezuela (Castaneda-Ruiz et al. 2003).
GenBank numbers – ZHKUCC 22-0008—OM780294 (LSU), OM780298 (SSU), OM780284(ITS) ZHKUCC 22-0009—OM780295 (LSU), OM780306 (SSU), OM780286 (ITS)
Notes – Our two strains (ZHKUCC 22-008 and ZHKUCC 22-009) share similar characters with Wiesneriomyces laurinus (Heredia et al. 2000; Rajashekhar and Kaveriappa 2000; Suetrong et al. 2014; Pratibha et al. 2015; Tennakoon et al. 2021). The phylogeny also showed that our strains (ZHKUCC 22-008 and ZHKUCC 22-009) clustered with other Wiesneriomyces laurinus strains (closer to BCC18609), with moderate statistical support (Fig. 2). This is the first report of Wiesneriomyces laurinus on Dimocarpus longan.
Figure 1 – Wiesneriomyces laurinus (ZHKU 22–0008, new host record) a–c Appearance of sporodochia on host. d Squash mount of sporodochium. e, f Conidiophores with setae. g–i Conidia. j Colonies on PDA. Scale bars: d=100 µm, e, f=20 µm, g, i=25 µm
Figure 2 – The best scoring RAxML tree for Wiesneriomycetaceae, for combined dataset of LSU, SSU and ITS sequence data. The tree is rooted with Bezerromyces brasiliensis (URM7411) and Bezerromyces pernambucoensis (URM7412). Ex-type strains are in bold and newly generated sequences are in red. Bootstrap support values for ML≥50% and BYPP≥0.95 are given above the nodes