Wicklowia submersa Boonmee, Sorvongxay & K.D. Hyde, Phytotaxa 411(1): 76 (2019)

Index Fungorum number: IF 556334; MycoBank number: MB 556334; Facesoffungi number: FoF 05994; Fig. 1

Saprobic on decaying wood submerged in a freshwater canal. Sexual morph Ascomata 93–186 × 126–179 μm (x̅=134×151 μm), immersed, erumpent when mature, raising the host tissue, dark ellipsoid or slit-like, uni-loculate, subglobose, dark brown, scattered, gregarious, ostiolate. Peridium 20–24 μm wide, thick-walled, composed of dark brown of pseudoparenchymatous cells of textura angularis. Hamathecium comprising 1–2 μm wide, numerous, cylindrical, filiform, branched, septate, hyaline pseudoparaphyses. Asci 64–95×21–28 μm (x̅=76×24 μm, n=7), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, apically thickened and rounded, with an ocular chamber when immature, sessile or short pedicellate. Ascospores 24–28×7–15 μm (x̅=26×9 μm, n=20), 2–3-seriate, partially overlapping, ellipsoidal-oblong to broad fusiform, with rounded ends, slightly inequilateral, asymmetrical, upper cell wider, hyaline, 1-septate, constricted at the septum, guttulate when immature, surrounded by a distinctly mucilaginous sheath, smooth-walled. Asexual morph Undetermined.

Culture characteristics – Ascospores germinated on PDA within 12 h. Colonies on MEA, reaching 0.5–1.5 cm diam., in 2 weeks at room temperature. Mycelium superficial, dark grey to brown, hairy, radiating outwards. effuse with wavy and dark brown edge.

Material examined – THAILAND, Uttaradit Province, Phichai, Thasak, Chom Tok Village, on decaying submerged wood in a freshwater canal, 29 December 2018, S. Boonmee, CTU11 (MFLU 21-0069, new record), living culture, MFLUCC 21-0092.

GenBank numbers – LSU = MZ538568, TEF1-α=MZ567109.

Notes – Wicklowia submersa was reported from a freshwater habitat collected in Krabi Province, southern Thailand (Boonmee et al. 2019). Our new isolate of W. submersa (MFLUCC 21-0092) was collected from Uttaradit Province in northern Thailand. Our new collection (Fig. 1) share similar characters of ascomata, asci and ascospores identical to the ex-type strain W. submersa (MFLUCC 18-0373). Phylogenetic analysis indicates that our strain consistently clustered with the ex-type strain MFLUCC 18-0373 with 100% MLBS, 1.00 BYPP support (Fig. 2). We therefore, report our isolate MFLUCC 21-0092 as a new record of W. submersa in Thailand.

Figure 1 – Wicklowia submersa (MFLU 21-0069, new record). a Appearance of ascomata on substrate. b Cross section of ascomata. c Section of peridium. d Pseudoparaphyses. e–h Asci. i–k Ascospores (i and j=ascospores mounted in water, k=ascospore stained in Nigrosin reagent). l Germinated ascospore. m, n Culture on MEA from surface and reverse. Scale bars: b=100 μm, c–h, l=20 μm, i–k=10 μm

Figure 2 – Maximum likelihood phylogenetic tree based on a combined LSU and SSU sequence data of Wicklowia taxa (Wicklowiaceae) and related families. The tree is rooted with Delitschia chaetomioides (SMH 3253.2) and D. winteri (CBS 225.62). Bootstrap support values for maximum likelihood (MLBS, left) equal to or greater than 50% is given above the nodes. Bayesian posterior probabilities (BYPP, right) equal to or greater than 0.95 are given above the nodes. Ex-type strains are in bold and newly generated sequences are in blue