Trichophoma cylindrospora Magaña-Dueñas, Cano & Stchigel, sp. nov.

MycoBank number: MB 833526; Index Fungorum number: IF 833526; Facesoffungi number: FoF 11730;

Etymology. From Greek κυλινδρικό-, cylindrical, due to the shape of the conidia.

Hyphae hyaline to brown, septate, branched, thin-walled, smooth to tuberculate, 1.5 – 2 µm wide. Conidiomata pycnidial, brown to blackish brown, immersed to semi-immersed, solitary, scattered, subglobose to pyriform, 300 – 390 × 300 – 410 µm, ostiolate, setose. Conidioma wall 4 – 6-layered, 15 – 30 µm thick, covered by a mass of interwoven, brown to dark brown hyphae, followed by an outer layer of textura angularis, composed of brown to dark brown, flattened polygonal cells of 5 – 8 µm diam, incrusted with a dark brown to carbonaceous material around the neck; neck dark brown to carbonaceous, cylindrical, 130 –145 × 100 –145 µm, covered by brown to dark brown, septate, erect, nodose, thick-walled setae 100 –180 × 2 – 4.5 µm, tapering towards the apex, mainly disposed around the ostiole. Conidiophores absent. Conidiogenous cells phialidic, determinate, hyaline, smooth-walled, ampulliform, 3 – 5 × 8 –14 µm. Conidia 0 –1-septate, hyaline, smooth- and thin-walled long cylindrical, 18 – 20 × 2 – 3 µm, guttulate, with a narrowly flattened base and rounded at the end.

Culture characteristics — (7 d at 25 °C). Colonies on potato dextrose agar (PDA) reaching 24 mm diam, flattened, velvety, margins regular, yellowish white to reddish brown (M. 4A2 / 8D8; Kornerup & Wanscher 1978); reverse reddish brown to dark brown (M. 8E8 / 8F8), exopigment reddish brown to golden yellow (M. 8D8 / 5B7). Colonies on oatmeal agar (OA) reaching 40 mm diam, flattened, slightly floccose, margin regular, grey (M. 8F1); reverse grey (M. 8F1). Colonies on malt extract agar (MEA) 2 % reaching 25–29 mm diam, flattened, velvety to slightly floccose, margins lobate, dark brown to greyish yellow (M. 8F8 / 4C5); reverse greyish brown to greyish orange, with yellowish brown patches (M. 8F3 / 5B5 / 5F7). Cardinal temperatures for growing — Optimum 30 °C, maximum 37 °C, minimum 15 °C.

Typus. Spain, Castilla y León community, Riaza, from plant debris, 4 May 2018, I.A. Iturrieta-González (holotype CBS H-24327; cultures ex-type CBS 146340 = FMR 17550; ITS, LSU and tef-1α sequences European Nucleotide Archive LR732023, LR732024 and LR732025, MycoBank MB833526).

Notes — Based on the phylogenetic analysis of the ITS, LSU and tef-1α combined dataset, the closest relative of T. cylindrospora is Forliomyces uniseptata. Forliomyces uniseptata differs from T. cylindrospora in producing shorter and broader conidia (10 –15 × 5 – 8 µm vs 18 – 20 × 2 – 3 µm), which are brown-coloured when mature (hyaline in T. cylindrospora) (Phukhamsakda et al. 2016). Based on a megablast search of NCBIs GenBank nucleotide database, the closest hit using the LSU sequence was Forliomyces uniseptata MFLUCC 15-0765 (GenBank NG_059659, Identities = 814/827 (98 %), no gaps); the closest hits using ITS was Forliomyces uniseptata MFLUCC 15-0765 (GenBank NR_154006, Identities = 440/458 (96 %), 3 gaps (0 %)); and the closest hits using the tef-1α sequence was Forliomyces uniseptata MFLUCC 15-0765 (GenBank KU727897, Identities = 420/438 (96 %), no gaps).