Fig 30, 31
≡ Asterina styracis Theiss., Abh. K.K. Zool.-Bot. Ges. Wien 7(3): 41 (1913)
Epiphytes on the upper surface, forming 0.1–0.2 mm diam., blackened, circular areas. Superficial hyphae spreading over the leaf surface, with appressoria and scattered with superficial thyriothecia, with arrow-like setae, 40– 62μm long. Appressoria 6–8.5×4–5.5μm diam, circular, lateral and alternative, sparsely septate. Sexual state: Thyriothecia 400–520μm in diam., solitary, gregarious, superficial, easily removed from the host surface, black, opening with star-like fissures when mature. Upper wall comprising a thin layer of mostly neatly arranged dark cells, which are branched at the outer rim, from which hyphae strands develop, base poorly developed. Hamathecium usually lacking pseudoparaphyses, with vertical asci. Asci 71– 80 × 70–75 μm (x = 74×71μm, n = 10), 8-spored, bitunicate, fissitunicate dehiscence not observed, globose to subglobose, apical region of asci usually with a thick opaque region, ocular chamber not observed, not staining blue in IKI. Ascospores 32–38×17–22μm (x = 36× 20μm, n=10), overlapping, conglobate, 1-septate, brown when mature, slightly constricted at the septum, with guttules, slightly spinose. Asexual states: Unknown.
Material examined: BRAZIL, Leopoldo Rio Grande Do Sul, on surface of leaves of Styrax acuminatus (Styracaceae), 1908, no. 77 (S-F113938, isotype of Asterina styracis).
Fig. 1 Trichasterina styracis (isotype of Asterina styracis). a Herbarium packet. b Herbarium specimen with black colony spots. c Fungal colony on substrate. d Thyriothecia when viewed in squash mount showing arrangement of asci. e Section through thyriothecium. f Hyphae with appressoria. g Hyphae mostly with opposite branching. h Redrawing of setae (from Anauld 1918). i Arrangement of cells in upper wall of thyriothecium. j, k Asci. l, m Immature ascospores. m Mature germinating ascospore. Scale Bars: d, e=100μm, f, g=50μm, j, k=20μm, i, l–n= 10μm