Tiarosporella dactylidis K.M. Thambugala, E. Camporesi & K.D. Hyde, sp. nov.
Index Fungorum Number: IF550768
Etymology: Referring to the host genus Dactylis.
Saprobic on grasses in terrestrial habitats. Sexual morph: Ascomata 170–215 μm high × 160–250μm diam, pseudothecia, immersed to erumpent on host tissue, visible as black spots on host tissue, uniloculate, scattered or gregarious, globose to subglobose, ostiolate. Ostiole circular, central, papillate. Peridium up to 26–54 μm wide, broader at the base, comprising of 2 layers: outer layer of thin, small, brown to dark brown cells of textura angularis, inner layer of thick, large, hyaline to lightly pigmented, cells of textura angularis. Hamathecium comprising 2–3 μm wide, hyphae-like, hyaline, sparse pseudoparaphyses. Asci 80–160 × 17–21 μm (= 107 × 19.2 μm, n = 15), 8-spored, bitunicate, fissitunicate, clavate to cylindro-clavate, pedicellate, apically rounded with an ocular chamber. Ascospores 22.5–28 × 7–8.5 μm ( = 25.2 × 8 μm, n = 25), uni to bi-seriate in the upper half, uniseriate at the base, hyaline, aseptate, ellipsoidal to fusiform, usually wider in the middle, thick-walled, smooth-walled, surrounded by a mucilaginous sheath. Asexual morph: Conidiomata up to 500 μm diam, on pine needles, solitary or in groups, scattered, globose to sub globose, dark brown to black, immersed to semi-immersed on PDA, superficial on pine needles, pulvinate, unilocular, ostiolate. Conidiomatal wall several cell layers thick, with outer layers composed of dark-brown cells of textura angularis, becoming thin-walled and hyaline towards the inner region. Conidiophores up to 7 μm long, cylindrical, hyaline, unbranched. Conidiogenous cells 6.2–10 × 1.2–2.5 μm (= 7.8 × 1.8 μm, n = 15), formed from the cells lining the inner walls of the conidiomata, phialidic, fusiform to cylindrical, determinate, hyaline. Conidia 2–4 × 1–2.2 μm (= 2.7 × 1.4 μm, n = 40), solitary, ovoid, straight, oval to ellipsoidal, producing conidia at their tips, smooth, hyaline, aseptate.
Culture characteristics: Colonies on PDA, covering 90 mm diam. petri dishes after 4 d in the dark at 25°C; circular, initially white, after 1 week becoming greyish brown to black; reverse grey to dark grayish green, white in the margin; flattened, fluffy, fairly dense, aerial, smooth surface with crenate edge, filamentous and conidia produced on pine needles after 3 weeks at 18°C.
Material examined: ITALY. Teodorano – Meldola (province of Forlì-Cesena