Sterigmatospora layueensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov.

MycoBank number: MB 828811; Index Fungorum number: IF 828811; Facesoffungi number: FoF; Figs 11C and 14O.

Etymology: the specific epithet layueensis refers to the geographic origin of the type strain, Layue county, Tibet.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.8 – 3.5 × 3.8 – 5.9 μm and single, budding is polar (Fig. 14O), a sediment is present. One or more conidia are produced on each stalk-like conidiophore. Conidiophore is single or multiple, usually cluster on cells. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is pale yellow, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudo- hyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose, sucrose, maltose, cellobiose, trehalose, raffinose, melezitose, D-xylose (variable), L- arabinose (variable), ribitol (variable), D-mannitol, D-glucitol, Methyl-α-D-glucoside and salicin (variable) are assimilated as sole carbon sources. Galactose, lactose, melibiose, inulin, soluble starch, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite is not assimilated. Maximum growth temperature is 20 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose- yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Layue county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5817T preserved in a metabolically inactive state, ex-type CBS 15649 = XZ100A2B).

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.

Fig. 14.