Spegazzinia camelliae N. Suwannarach, J. Kumla & S. Lumyong, Phytotaxa 483(2): 120 (2021)
Index Fungorum number: IF 837969; MycoBank number: MB 837969; Facesoffungi number: FoF 09467; Fig. 1
Saprobic on decaying wood. Sexual morph Undetermined. Asexual morph Hyphomycetous. Colonies punctiform, effuse, scattered, dark brown to black on natural wood substrate. Mycelium slight raised on host surface, branched, septate, hyaline to pale brown. Conidiophores micronematous, mononematous, basauxic, slight erect, flexuous, simple, unbranched, intercalarily, pale brown. Conidiogenous cells indiscrete, indeterminate, terminal. Conidia 14–17 diam. µm, holoblastic, globose to subglobose, 4-celled, cruciately septate and constricted at the septa, dentate at the margin, brown to dark brown, darker at the septa, faint septa at maturity, guttulate when immature, smooth-walled.
Culture characteristics – Conidium germinating on PDA within 24 h and germ tubes arising from terminal end. Colonies on PDA, slow growing, reaching 5 cm diam. after 1 month at room temperature, flat to slightly effuse, surface smooth, circular, radial striations and entire edges, olivaceous brown to dark brown, and sporulated conidia in cultures after 14 days. Mycelium superficial, partly immersed, composed of branched, septate, hyaline to pale brown, smooth-walled. Conidia 11–17 µm diam., globose to subglobose, 4-celled cruciate septate and constricted at the septa,
dentate at the margin, brown to dark brown, darker at the septa, guttulate, smooth-walled.
Material examined – THAILAND, Phitsanulok Province, Wang Thong, Wang Nok Aen, on decaying wood, 25 July 2019, S. Boonmee, WNA03 (MFLU 21-0053, new record), living culture, MFLUCC 21-0076.
GenBank numbers – ITS=MZ538526, LSU=MZ538560, TEF1-α=MZ567102.
Notes – Spegazzinia camelliae was described as an endophytic isolate from living leaves of the Camellia sinensis plant in northern Thailand (Suwannarach et al. 2021). Based on phylogenetic analysis of a combined LSU, ITS and TEF1-α sequence dataset of Spegazzinia species, our strain MFLUCC 21-0076 clusters with the ex-type strain SDBRCMU328 with 88% MLBS, 1.00 BYPP support (Fig. 2). When compared our strain was similar with the type specimen of S. camelliae (SDBR-CMU328), they are morphologically similar (Fig. 28). This is the first record of S. camelliae (MFLU 21-0053) as a saprobic strain on decaying wood.
Figure 1 – Spegazzinia camelliae (MFLU 21-0053, new record). a Colonies on wood substrate. b, c Conidiophores and conidiogenous cells. d–h Conidia. i Germinated conidium. j, k Culture on PDA from surface and reverse at 1 month. l–r Conidia developing in culture. Scale
bars: b–c, i, l=20 µm, d–f, n–p=5 µm, g–h, m, q–r=10 µm
Figure 2 – Phylogram generated from maximum likelihood analysis based on combined LSU, ITS and TEF1-α sequence data of Spegazzinia taxa. Verrucoconiothyrium nitidae (CPC1532 and CPC25373) are selected as the outgroup taxa. Bootstrap support values for maximum likelihood (MLBS, left) equal to or greater than 70% is given above the nodes. Bayesian posterior probabilities (BYPP, right) equal to or greater than 0.95 are given above the nodes. Ex-type strains are in bold and newly generated sequence is in blue