Robertozyma ningxiaensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov.
MycoBank number: MB 828826; Index Fungorum number: IF 828826; Facesoffungi number: FoF. Fig. 15O.
Etymology: the specific epithet ningxiaensis refers to the geographic origin of the type strain, Ningxia province, China.
Culture characteristics: In YM broth, after 7 d at 17 °C, cells are oval and ellipsoidal, 3.2–4.5 × 3.9–6.8 μm and single, budding is polar (Fig. 15O), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange red, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.
Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), trehalose, glycerol, D-mannitol, salicin (delayed and weak) and succinate are assimilated as sole carbon sources. L-sorbose, sucrose, maltose, cellobiose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, erythritol, ribitol, galactitol, D- glucitol, Methyl-α-D-glucoside, D-gluconate, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine and ethylamine hydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 22–23 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.
Typus: China, Helanshan mountain, Ningxia province, obtained from soil, Aug. 2009, P.J. Han (holotype CGMCC 2.4451T preserved in a metabolically inactive state, ex-type CBS 12499 = HLS10.23).
Fig. 15.