Purpurepithecium murisporum Jayasiri & K.D. Hyde sp. nov.                               Fig. 1–2          

            Holotype: MFLU 16-2983

Index Fungorum number: IF553001; Facesoffungi number: FoF 03110

Etymology: The species epithet “murisporum” refers to the muriform ascospores

Saprobic on scale of pine cones on ground. Sexual morph:  Ascomata hysterothecia 228–320 × 260–293 × 700–1000 µm ( = 278 × 272 × 850 µm, n = 10), erumpent to superficial, with a prominent longitudinal slit, scattered to gregarious, sometime overlap each other, navicular, flexuous. Epithecium 2–2.5 μm wide, dense, cellular, swollen tip, branched, pigmented with purple. Peridium 28–62 µm ( = 41 µm) wide, narrower at base within the substrate, widest at mid-point, carbonaceous and brittle when dry. Pseudoparaphyses cellular, septate, persistent, 1–1.5 μm wide, hyaline in mass, branched above. Asci 107–157 × 23–30 µm ( = 124 × 25 µm, n = 30), cylindrical to clavate, bitunicate, short-stipitate. Ascospores 30–37× 10–13 µm ( = 32 × 11 µm, n = 30), asymmetric, when young, mature symmetric, hyaline to dark brown, transversely septate or muriform, with curved ends, septation highly variable, usually 7–10 longitudinal and many vertical septa, prominently constricted at the median septum, gelatinous sheath absent. Asexual morph: Hyphomycetes. punctiform, pulvinate, granular, black, shining. Mycelium 1.5–4.5 μm immersed in the media, composed of branched, septate, smooth, subhyaline to pale brown, hyphae. Conidiophores 25–30 × 3–4.5 μm, pale brown, short, slender, articulated. Conidiogenous 6–9 × 5–7 µm ( = 8 × 6 µm, n = 10), hyaline, cells integrated, terminal, determinate. Conidia 14–24 × 10–14 µm ( = 19 × 12 µm, n = 10), solitary, irregular, clavate, compact, two cells to muriform, 3–4 rows of transverse septa, constricted at the septa, dark and thickly banded at the septa. The number of cells per conidium varies from 2 to 11, reddish brown to brown, smooth, basal cell 4–6 × 2–4 μm, subhyaline to pale brown, cuneiform, with thinner wall.

Material examined: THAILAND, Chiang Mai Province, on decaying scales of a pine cone, 22 July 2015, Subashini C. Jayasiri C 095 (MFLU 16-2983, Holotype), PDD (Isotype), living culture MFLUCC 16-0611; BCC; ibid., on decaying scales of pine cone, 23 December 2015, Subashini C. Jayasiri C 95-B (MFLU 17-0447, Paratype), living culture 17-0319. GenBank no: MFLUCC 16-0611- LSU KY799173, SSU KY887665, RPB2 KY799176 TEF1 KY887666; MFLUCC 17-0319 – LSU KY799174, SSU KY799175, RPB2 KY887667, TEF1 KY799177

Culture characteristics: Ascospores germinating on MEA within 24 h. Colonies growing on MEA 2 cm diam. after 30 days at 18 C, very slow growing, after this no more growth happen, chlamydospores formed, then asexual structures formed in culture. colonies circular, effuse, dense, dark brown, diffuse into media, many layers, smooth surface with entire to slightly undulate edge with brown yellow pigment (Fig. 2a, b).

Fig. 1. Purpurepithecium murisporum (holotype). a A pine cone.  b, c View of hysteriothecia on host surface. d Section through hysteriothecium. e Peridium. f Pseudoparaphyses. g-j Ascospores. k Germinated ascospores.  l–o Asci. Scale bars: d = 100 µm, e = 20 µm, f = 10 µm, g–k = 10 µm, l–p = 30 µm.

 

Fig. 2. Purpurepithecium murisporum asexual morph from culture. a, b Forward and reverse view of the culture. c, d Appearance of fruiting bodies in culture. e–h Conidiophores with conidia. i, j Conidiogenous cells. k, l Conidia.  Scale bar: a, b = 2 cm, c, d = 200 µm, e–k = 20 µm, l = 30 µm.