Purpureofaciens W. Dong, H. Zhang & K.D. Hyde, in Dong et al., Fungal Diversity: 10.1007/s13225-020-00463-5, [55] (2020)

Index Fungorum number: IF557804; Facesoffungi number: FoF08717

Etymology: referring to staining the substrate purple

Saprobic on decaying wood submerged in freshwater.

Sexual morph: Ascomata black, scattered or gregarious, semi-immersed to superficial, conical, flattened at the basal region, stain the substrate purple and produce reddish pigmentation at the apex, coriaceous, with a large, protuberant, ostiolate papilla. Peridium of unequal thickness, poorly developed at the base, composed of reddish brown, large cells of textura angularis. Pseudoparaphyses numerous, trabeculate, filiform, hyaline, embedded in a hyaline gelatinous matrix. Asci 8-spored, bitunicate, cylindrical, pedicellate, apically rounded. Ascospores uniseriate, curved, olivaceous, septate, ellipsoidal with acute ends, guttulate, thin-walled, smooth, surrounded by a thin mucilaginous sheath.

Asexual morph: Undetermined.

Type species: Purpureofaciens aquatica W. Dong, H. Zhang & K.D. Hyde

Notes: – Purpureofaciens is unique by semi-immersed to superficial, conical ascomata staining woody substrate purple, cylindrical asci, ellipsoidal, curved, olivaceous ascospores with acute ends and white to reddish colonies in culture (Mugambi and Huhndorf 2009; Liu et al. 2015). The reddish culture is also observed in a strain of Anteaglonium parvulum (MFLUCC 14-0821), which has a similar shape to ascospores, but the characters of ascomata, asci and ascospores can easily separate them (Jayasiri et al. 2016). Flammeascoma has reddish pigmentation at the apex of ascomata, which resembles Purpureofaciens. However, the conical ascomata with a large, protuberant, ostiolate papilla, cylindrical asci and ellipsoidal, olivaceous ascospores of Purpureofaciens are different from those of Flammeascoma (Liu et al. 2015). Purpureofaciens clusters with Flammeas- coma species with low bootstrap support (Fig. 2) and morphology separates them as distinct genera. Purpureofaciens comprises one freshwater species P. aquatica which was collected from Thailand.

Fig. 2 Phylogram generated from maximum likelihood analysis of combined LSU, 100/1.00    Periconia pseudodigitata KT 644 100/1.00    Periconia pseudodigitata KT 1195A F ITS, TEF and RPB2 sequence data for species of Dothideo- mycetes. Bootstrap values for maximum likelihood equal to or greater than 75% and Bayesian posterior probabilities equal to or greater than 0.95 are placed near the branches as ML/BYPP. Newly generated sequences are in red and ex-type strains are in bold. The new species intro- duced in this study are indicated with underline. Freshwater strains are indicated with a red letter “F”. Orders not treated in this study are compressed. The tree is rooted to Capronia pilo- sella AFTOL-ID 657 and Endo- carpon pallidulum AFTOL-ID 661 (Eurotiomycetes)

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