Purpureofaciens aquatica W. Dong, H. Zhang & K.D. Hyde, sp. nov.

Index Fungorum number: IF557902; Facesoffungi num- ber: FoF08718; Fig. 26

Etymology: in reference to aquatic habitat of the fungus

Holotype: MFLU 18-1569

Saprobic on decaying wood submerged in freshwater. Sexual morph: Ascomata 200–300 μm high, 300–400 μm diam., dark brown to black, scattered or gregarious, semi- immersed to superficial, conical, flattened at the basal region, stain the substrate purple and produce reddish pig- mentation at the apex, coriaceous, with a large, protuber- ant, ostiolate papilla, 110–130 μm long, 110–130 μm wide. Peridium unequal thickness, poorly developed at the base, up to 45 μm at the sides and 15 μm at the base, composed of reddish brown, large cells of textura angularis. Pseudopara- physes 2 μm diam., numerous, trabeculate, filiform, hyaline, embedded in a hyaline gelatinous matrix. Asci 135–150 × 8.5–11 μm (x̄ = 143 × 9.7 μm, n = 10), 8-spored, bitunicate, cylindrical, pedicellate, apically rounded. Ascospores 15–22 × 7–10 μm (x̄ = 18.5 × 8 μm, n = 20), uniseriate, curved, olivaceous, 1-septate, constricted at the septum, ellipsoidal with acute ends, with two prominent guttules when imma- ture, thin-walled, smooth, surrounded by a thin mucilagi- nous sheath. Asexual morph: Undetermined.

Culture characteristics: On PDA, colony circular, reach- ing 50 mm diam. in 25 days at 25 °C, annulate, white to reddish to purplish red from center to edge, surface rough, dry, raised, edge entire.

Material examined: THAILAND, Satun Province, on submerged wood in a stream, 10 May 2018, W. Dong, hat266-1 (MFLU 18-1569, holotype), ex-type living cul- ture MFLUCC 18-1241; ibid. hat266-2 (HKAS 105016, isotype), ex-type living culture KUMCC 19-0032.

Fig. 26 Purpureofaciens aquatica (MFLU 18-1569, holotype). a–c Appearance of black ascomata on host surface. d, e Vertical section of ascomata. f Structure of peridium. g Pseudoparaphyses. h, i Bituni- cate asci. j–l Ascospores. m Germinated ascospores. n, o Colony on PDA (left-front, right-reverse). Scale bars: d, e = 100 μm, fi, m = 20 μm, jl = 10 μm

Species