Pleopunctum ellipsoideum N.G. Liu, K.D. Hyde & J.K. Liu, in Liu et al., Mycosphere 10(1): 767 (2019).

Index Fungorumnumber: IF 556523; MycoBank number: MB 556523; Facesoffungi number: FoF06114; Fig. 1

Saprobic on decaying wood. Asexual morph: Hyphomycetous. Colonies on natural substrate forming sporodochial conidiomata, superficial, black, scattered, velvety, glistening, orbicular. Mycelium immersed, composed of branched, septate, subhyaline to pale brown hyphae. Conidiophores and conidiogenous cells not observed. Conidia 33–45×16–20 μm (x̄ =38×18 μm, n=35), acrogenous, solitary, oval to ellipsoidal, muriform, constricted at septa, yellowish brown to dark brown, broadly obtuse at apex, truncate at base. Basal cell 6.5–10.5×9.5–11.5 μm (x̄ =9–10.5 μm, n=35), hyaline, elliptical to subglobose, smooth walled. Sexual morph: Not observed. Culture characteristics: Conidium germinated on PDA within 12 h. Colonies on PDA reaching 20 mm in 4 weeks at 26 °C. Mycelia superficial, circular, entire, flat, rough, grey brown from above, dark brown from below.

Material examined – Thailand, Chiang Mai Province, Mae Taeng District, MRC, on bamboo culms, 15 July 2020, Y.R. Sun, M10 (MFLU 21–0091); living culture, MFLUCC 21–0064.

Hosts – decaying wood (Liu et al. 2019a, b, c, d) and bamboo (this study)

Distribution – China (Liu et al. 2019a, b, c, d) and Thailand (this study)

GenBank numbers – OM250079 (ITS), OM258687 (LSU)

Notes – Pleopunctum ellipsoideum was isolated from decaying wood in China by Liu et al. (2019a, b, c, d). The morphological characters of our collection are the same as in P. ellipsoideum (MFLUCC 19-0390). Phylogenetic analysis based on a combined LSU, ITS and tef1 sequence data indicated that our isolate and P. ellipsoideum (MFLUCC 19–0390) clustered together with high support (ML 100% and 0.99 BYPP; Fig. 2). Based on both morphology and phylogeny, we identified our taxon as P. ellipsoideum. This is the first geographical and host report of P. ellipsoideum on bamboo in Thailand.

Figure 1 – Pleopunctum ellipsoideum (MFLU 21–0091, new record) a, b Colonies on natural substrates c–i Conidia with basal hyaline cells j Germinated conidium k, l Colonies on PDA media. Scale bars: a=500 μm, b=200 μm, c=50 μm, d–j=20 μm

Figure 2 – Phylogram generated from maximum likelihood analysis based on combined LSU, ITS and tef1sequence data of Phaeoseptaceae. Bootstrap support values for ML≥than 75% and BYPP≥0.95 are given above the nodes. The ex-types are in bold; the new isolates are in blue. The tree is rooted with Amorosia littoralis (NN 6654) and Angustimassarina populi (MFLUCC 13–0034)