Phaeosphaeriopsis glaucopunctata (Grev.) M.P.S. Câmara et al.
≡ Cryptosphaeria glaucopunctata Grev., Fl. Edin.: 1-478 (1824)
Saprobic on Ruscus aculeatus (Asparagaceae). Sexual state: Ascomata 110–145 µm high, 130–220 µm diam, scattered, solitary, immersed, visible as small, brown spots surrounded by pale yellowish region, uniloculate, subglobose, glabrous, with central black ostiole penetrating through host surface. Peridium 7–18 µm wide, thin-walled, of unequal thickness, composed of 3–4 layers of thickened, brown, pseudoparenchymatous cells, arranged in a textura angularis. Hamathecium composed of sparse, 1–3 µm wide, filiform, frequently anastomosing, broad cellular pseudoparaphyses, with distinct septa, constricted at the septa. Asci (55–)60–75(–76) × (11–)12–14(–15) µm ( = 68.2 × 13.4 μm, n = 20), bitunicate, 8-spored, broadly cylindrical to cylindric-clavate, subsessile to short pedicellate, apically rounded with indistinct ocular chamber. Ascospores 18–20(–22) × (3–)4–5.5 µm ( = 20 × 4.8 μm, n = 20), overlapping 1–3-seriate, phragmosporous, cylindrical, narrower and longer at the lower cell, brown to dark brown, 4-septate, often enlarged at the forth cell, rough-walled, echinulate, surrounded by mucilaginous sheath. Asexual state: Conidiomata pycnidial, scattered, solitary, immersed to superficial (in agar), uniloculate, globose, glabrous, brown to black, ostiolate, with black droplets. Conidiomata walls thin, comprising 2−3 layers of yellowish-brown to brown, pseudoparenchymatous cells, arranged in textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells holoblastic, phialidic, annellidic, with a short collarette, ampulliform to subcylindrical, hyaline, lining the inner cavity of conidioma. Conidia amerosporous, cylindrical to oblong, with rounded ends, hyaline to yellow, becoming yellowish-brown at maturity, aseptate, smooth to finely verruculose, accumulating in a huge bluish-black slimy mass at the tip of ostiole (from Thambugala et al. 2014).
Material examined: UK, near Edinburgh, Slateford, on dead leaves of Ruscus aculeatus L. (Asparagaceae), 15 August 1824, R.K. Greville (E 00074283, syntype).
Fig. 1 Phaeosphaeriopsis glaucopunctata (E00074283, syntype). a Herbarium label and specimens of Phaeosphaeriopsis glaucopunctata. b Ascomata on host surface. c Section through ascomata d Section through peridium. e Asci with pseudoparaphyses. f Cellular pseudoparaphyses. g Ascus. h–i Asci stained by KOH reagent. j–o Ascospores. Scale bars: c = 100 µm, d–i = 20 µm, j–o = 5 µm.
