Pestalotiopsis linguae Li Hua, Manawas., Maharachchi., and K.D.Hyde sp. nov.

Index Fungorum number:IF; MycoBank number: MB; Facesoffungi number:  FoF 12712;

Holotype: ZHKUCC 22-0159

Endophytic on the Pyrrosia lingua (Polypodiaceae) leaves. Sexual morph: not observed. Asexual morph: Conidiomata acervular, 800–1100 μm diameter, globose, solitary or aggregated in clusters, exuding black conidial masses. Conidiophores reduced to conidiogenous cells. Conidiogenous cells discrete or integrated, cylindrical to subcylindrical, hyaline, smooth, annelidic. Conidia (16.5–)18–21(–23) × (4–)5–7(–8) μm (x̅ ± SD = 19.2 ± 1.4 × 5.9 ± 0.8 μm, n = 30), fusoid, ellipsoid, straight or slightly curved, 4-septate; basal cell conical, (2–)3–5 μm, hyaline, smooth, thin-walled; with a single appendage (3–)4–7(–8) μm (x̅ ± SD = 5 ± 1.3 μm, n = 30) long, filiform, unbranched, centric; three median cellsdoliiform, (11–)11.5–13(–14) μm (x̅ ± SD = 12.2 ± 0.7 μm, n = 30) long, smooth, concolorous, brown, septa darker than the restof the cell (second cell from base (3–)4–5 μm long; third cell (2–)3–4(–5) μm long; fourth cell (3–)4–5 μm long); apical cell conical, (2.5–)3–4(–4.5) μm (x̅ ± SD = 3.7 ± 0.5 μm, n=30) long, hyaline, smooth, thin-walled; with 2–3 apicaltubular appendages, unbranched, filiform, (10–)12–17(–21.5) μm (x̅ ± SD = 14.9 ± 2.5 μm, n = 30) long.

Culture characteristics – Colonies on PDA reaching 50 mm diameter after five days at 25 ℃. Colonies filamentous to circular, medium dense, white aerial mycelium on surfaceflat or raised.

Material examined – CHINA, Guangdong Province, Guangzhou City, from leaf of Pyrrosia lingua (Thunb.) Farwell, 16 August. 2021, Li Hua (holotype ZHKUCC 22-0159, new species).

Fig. ** Pestalotiopsis linguae (ZHKUCC 22-0159, holotype) a. Colony on PDA after 5 days at 25℃—surface view and reverse. b. Conidioma formed on PDA. c-d. Conidiophores, conidiogenous cells and attached conidia. e-g. Conidia. Scale bars: 10 μm.