Melanconis Tul. & C. Tul., Select. fung. carpol. (Paris) 2: 115 (1863)

Index Fungorum number: IF 3060; 36 morphological species (Species Fungorum 2020), 8 species with sequence data.

Notes – Melanconis was introduced by Tulasne & Tulasne (1863) with the type Melanconis stilbostoma. The asexual morph of Melanconis is placed in Melanconium, and both of these genera have been linked (Maharachchikumbura et al. 2016). Species in both Melanconis and Melanconium cause diseases such as cankers and shoot blights on plants (Maharachchikumbura et al. 2016). The genus is characterised by ascomata with 8-spored asci, ellipsoid, 1-septate ascospores, and acervular conidiomata with hyaline to brown, ellipsoid or subglobose conidia. Most of the species are known in North America and Europe (Senanayake et al. 2017a). There are 113 species epithets listed in Index Fungorum (2020). However, sequence data is available for only six species in the GenBank. Placement of our isolates in the genus is provided.

Fig 1 – Phylogram generated from maximum likelihood analysis based on combined LSU, and ITS sequence data representing family Melanconidaceae. Related sequences are taken from Fan et al. (2018). Ten strains are included in the combined analyses which comprise 1720 characters (1200 characters for LSU, 520 characters for ITS) after alignment. Gnomonia gnomon (CBS 119.53) is used as the outgroup taxon. For both genes, GTR+G was applied as the evolutionary model. The best RaxML tree with a final likelihood value of – 3656.825130 is presented. The matrix had 164 distinct alignment patterns, with 21.37%undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.264911, C = 0.220911, G = 0.264143, T = 0.250035; substitution rates AC = 1.561251, AG = 2.163552, AT = 2.584612, CG = 0.506469, CT = 4.714002, GT = 1.000000; gamma distribution shape parameter α = 0.808825. Bootstrap values for ML equal to or greater than 75% (first set) and MP equal to or greater than 75% (second set) are given above the nodes. Ex-type strains are in black and the new strain is indicated in yellow.