Manoharachariella tectonae Doilom & K.D. Hyde, sp.nov.

MycoBank number: MB 551972; Index Fungorum number: IF 551972;Facesoffungi number: FoF 01865;

Etymology: Name refers to the host genus Tectona on which the fungus was collected.

Holotype: MFLU 15–3413

Saprobic on dead branches of T. grandis. Sexual morph: Undetermined. Asexual morph: Colonies on natural substrate, superficial, solitary, scattered, dark brown. Conidiophores micronematous. Conidia (45–)50–54(−65) × (17–)20–23(−26) μm (x = 52 × 22 μm, n = 20),subhyaline when immature, becoming dark blackish-brown in center part of mature conidia, hyaline to subhyaline at 1–2 tiers of apical and basal cells, doliiform, obpyriform, ellipsoidal, dictyoseptate, solitary, apiculate, 6–10 longitudinal septa, and 7–11 transverse septa, dark brown at the septa; terminal cells hyaline to subhyaline, 6–12 μm long, 5–7 μm wide, rounded at the apex, thick-walled.

Culture characteristics: Ascospores germinating on PDA within 24 h. Colonies on MEA reaching 11–16 mm diam. after 14 days in the dark at 25 °C, (x = 14.2 mm, n = 5), edge entire to erose or dentate, sometimes fimbriate, flat or effuse at the edge, raised at the old mycelium plugs in the center, velvety, colonies light brown (5D4) at the center, brown (6F4) at the edge, brownish grey (6F2) at the center and grayish brown (7E3) at the edge from below.

Habitat: Known to inhabit dead branches of T. grandis (current study).

Known distribution: Thailand (current study).

Material examined: THAILAND, Phrae Province, Denchai District, Ban Maejour Subdistrict, on dead branches of T. grandis, 29 October 2011, M. Doilom, (MFLU 15–3413, holotype), ex-type living culture MFLUCC 12–0170, MKT 017, ICMP 21153, GenBank Accession No: ITS: KU144935, LSU: KU764705, TEF1: KU872762.

Notes: The genus Manoharachariella presently consists of three species according to Index Fungorum (2016), with M. lignicola as the type species. Manoharachariella tectonae (MFLU 15–3413) was compared with the three species (Table 8). Conidia of M.tectonae are longer and wider than M. elsadii but shorter and narrower than M. indica, longer and narrower than M. lignicola (Bagyanarayana et al. 2009) (Table 8). Conidia of M. tectonae are somewhat similar to the generic type, but conidial septation is 7–11 transverse septa while there are 7–9 transverse septa in M. lignicola (Table 8). Based on morphological features of the conidia size and shape as well as number of transverse septa this specimen is recognized as new species.

FIG Manoharachariella tectonae (MFLU 15–3413, holotype). a Conidia on host substrate. b–g Conidia with apiculi. h Colony on PDA after 6 months (above and below views). Scale bars: a = 200 μm, b, c = 20 μm, d–g = 10 μm