Lophiotrema hydei J.F. Zhang, J.K. Liu & Z.Y. Liu, Phytotaxa 379(2): 172 (2018)
Index Fungorum number: IF 555509; MycoBank number: MB 555509; Facesoffungi number: FoF 05216; Fig. 1
Saprobic on wood of Rosa sp. Sexual morph: Ascomata 220–300 × 200–250 μm (x̅=261 × 229 μm, n=10), solitary or gregarious, immersed to erumpent through host tissue, globose to subglobose, brown to dark brown,
uni-loculate, glabrous, ostiolate. Ostiole mostly central. Peridium 20–40 μm wide, composed of angular cells, outer layer, dark brown, thick-walled cells, inner layer, hyaline with thin-walled cells. Hamathecium composed of numerous, 1.5–2.5 µm wide, filamentous, septate, branched, cellular pseudoparaphyses. Asci 80–120 × 10–13 µm (x̅=105 × 11 µm, n=20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate, apically rounded, with a minute ocular chamber. Ascospores 25–35×4–7 µm (x̅=31×5.5 µm, n=30), overlapping biseriate, fusiform, hyaline, straight or slightly curved, 1-septate, the upper cell is longer than the lower cell, deeply constricted at the septum, narrower towards both ends, smooth-walled, guttulate. Asexual morph: Undetermined.
Culture characteristics – Ascospores germinating on PDA within 24 h and germ tubes arising from both ends. Colonies on PDA, slow growing, reaching 2 cm diam. after 4 weeks of incubation at room temperature, initially white becoming blackish brown at maturity, slightly effuse, radially with an undulate edge, reverse blackish brown.
Material examined – CHINA, Yunnan, Diqing Autonomous Prefecture, Shangri-La, Xiaozhongdianzhen (27.468883°N 99.845828°E), 2958 m, on dead wood of Rosa sp. (Rosaceae), 30 August 2020, GC Ren, DQ33
(HKAS 115782, new host record), living culture could not maintain.
GenBank numbers – ITS = MZ493297, LSU = MZ493311, SSU = MZ493283, TEF1-α = MZ508406.
Notes – Zhang et al. (2018) introduced Lophiotrema hydei as a saprobe on herbaceous plant from Guizhou Province, China. In this study, we found a new strain that grouped with the ex-type strain of L. hydei in multi-gene phylogenetic analysis with 98% MLBS support (Fig. 2). There were no nucleotide differences between these two strains in SSU, LSU, ITS and TEF1-α. The asci of the new isolate are comparatively larger (80–120 × 10–13 µm vs. 78–89(–99)×6.9–8.8 µm), but other features are similar in dimensions (Fig. 1). Therefore, we identify our collection as a new host record of Lophiotrema hydei on Rosa sp. from Yunnan Province, China.
Figure 1 – Lophiotrema hydei (HKAS 115782, new host record). a, b Appearance of ascomata on host surface. c Longitudinal section of an ascoma. d Section of the peridium cells. e Pseudoparaphyses. f–j Asci. k–o Ascospores. p, q Culture characteristic on PDA (p=from above, q=from below). Scale bars: c=100 μm, d, f–j=20 μm, e=5 μm, k–o=10 μm, p, q=10 mm
Figure 2 – Phylogram generated from maximum likelihood analysis based on combined SSU, LSU, ITS, TEF1-α and RPB2 sequence data to indicate the newly generated strains in Lophiotremataceae. Sixty strains are included in the combined analyses which comprise a total of 4357 characters. Murilentithecium clematidis (MFLUCC 14-0561 and MFLUCC 14-0562) is selected as the outgroup taxon. The best RAxML tree with a final likelihood value of − 24211.323363 is presented. RAxML analysis yielded 1272 distinct alignment patterns and 6.17% of undetermined characters or gaps. Estimated base frequencies were as follows: A=0.246394, C=0.256619, G=0.267068, T=0.229919, with substitution rates AC=1.576531, AG=4.38051, AT=1.49611, CG=1.339907, CT=10.502267, GT=1.000000; gamma distribution shape parameter alpha=0.450773. Bootstrap support values for maximum likelihood (MLBS, left) equal to or greater than 70% is given above the nodes. Ex-type strains are in bold and newly generated sequences are in blue