Lophiostoma clematidis-vitalbae Andreasen, Jaklitsch & Voglmayr, in Andreasen et al., Persoonia 46: 251 (2021)

Index Fungorum number: IF 838978; MycoBank number: MB 838978; Facesoffungi number: FoF 07294; Fig. 1

≡ Sigarispora clematidis Phukhams. & K.D. Hyde, in Phukhamsakda et al., Fungal Diversity 102: 53 (2020)

Saprobic on dead stem of unknown host. Sexual morph Ascomata 504–534 μm high, 372–473 μm diam. (x̅=523 × 413 μm, n=10), solitary or aggregated, semiimmersed to superficial, with only black shiny ostioles visible, globose, coriaceous, dark brown to black, rough-walled, ostiolate. Ostioles 165–213×25–98 μm (x̅=193×52 μm, n=5), with a crest-like apex and a pore-like opening, central, elongated and laterally compressed, irregular wall, filled with hyaline periphyses. Peridium 62–82 μm wide (x̅=70 μm, n=20), wider at the apex, thinner at the base, with lightly pigmented dark brown to black, thick-walled cells of textura angularis, cell towards the inside lighter, somewhat flattened. Hamathecium composed of numerous, dense, 1.8–2.3 µm wide, filamentous, septate, pseudoparaphyses. Asci 93–111×11–14 µm (x̅=101×12 µm, n=30), 8-spored, bitunicate, fissitunicate, clavate, with furcate pedicel, rounded at the apex, with an ocular chamber. Ascospores 19–25×5–7 µm (x̅=23×6 µm, n=50), bi-seriate or partially overlapping, broad fusiform, tapering towards the ends, initially hyaline, becoming yellowish brown at maturity, acute ends, mostly curved, 1–5 transversely eusepta, slightly constricted at the septa, cells above central septum swollen, a guttulate, with 5–9 µm long sheath drawn out to form polar appendages. Asexual morph Undetermined.

Material examined – UZBEKISTAN, Jizzakh Province, Zaamin District, Zaamin National Nature Park, river of Ettisuv, Turkestan range of Pamir-Alay Mountains, on dead stem of Lonicera sp. (Caprifoliaceae), 10 July 2019, Y. Gafforov and T. Kholmuradova YG-Z32-2 (TASM 6157, new host and geographical record).

GenBank numbers – ITS = MZ576206, LSU=MZ577239, SSU=MZ577241, TEF1-α=MZ605437, RPB2=MZ605438.

Notes – In phylogenetic analysis of Lophiostoma, our strains (TASM 6157) clusters with the ex-type strain Lophiostoma clematidis-vitalbae (MFLUCC 16-1368) with 0.98 BYPP (Fig. 2). Our strain (Fig. 1) shares similar ascomata, asci and ascospores features identical to the type strain L. clematidis-vitalbae (MFLUCC 16-1368) (Phukhamsakda et al. 2020). Therefore, L. clematidis-vitalbae is reported as a new host and geographical record on Cousinia umbrosa for Uzbekistan.

Figure 1 – Lophiostoma clematidis-vitalbae (TASM 6157, new host and geographical record). a–c Appearance of ascomata on host surface. d Vertical section of ascoma. e Ostiolar canal. f Periphyses at ostiole. g Pseudoparaphyses. h Peridium. i–m Asci. n–q Ascospores. Scale bars: d, e=100 µm, f–h=50 µm, i–m=20 µm, n–q=10 µm

Figure 2 – Phylogram generated from maximum likelihood analysis based on combined SSU, LSU, ITS, TEF1-α and RPB2 sequence data for Lophiostomataceae. One hundred twelve strains are included in the combined analyses which comprise a total of 4199 characters. Teichospora rubriostiolata TR7 and Teichospora trabicola C134 are selected as the outgroup taxa. The best RAxML tree with a final likelihood value of − 30611.078207 is presented. RAxML analysis yielded 1567 distinct alignment patterns and 25.77% of undetermined characters or gaps. Estimated base frequencies were as follows: A=0.248812, C=0.247504, G=0.267733, T=0.235951, with substitution rates AC=1.717311, AG=4.965346, AT=1.578028, CG=1.454739, CT=10.722777, GT=1.000000; gamma distribution shape parameter alpha=0.639152. Support values for maximum likelihood (MLBS, left) greater than 70% and Bayesian posterior probabilities (BYPP, right) greater than 0.90 are given at the nodes. Extype strains are in bold and newly generated sequences are in blue

Figure 2 – (continued)