Lindgomyces guizhouensis J. Mai, Y.Z. Lu & K.D. Hyde, sp. nov.

Index Fungorum number: IF 559506; MycoBank number: MB 559506; Facesoffungi number: FoF 10676; Fig. 1

Etymology – Referring to collecting site in Guizhou Province, China.

Holotypus – GZAAS 21–0383

Saprobic on decaying wood in terrestrial habitats. Asexual morph: Ascomata 396–548 μm high × 466–514 μm diam., superficial, black, smooth, scattered, solitary, erumpent, subglobose to broadly conical, with a centrally located ostiole. Peridium 44–79 μm thick, thick–walled, composed of pale brown cells of textura angularis. Hamathecium 1–2 μm wide, septate, branched, pseudoparaphyses above the asci. Asci 110–154×15–28 μm, bitunicate, fissitunicate, apically rounded with an indistinct ocular chamber, subsessile, broadly cylindrical to clavate. Ascospores 30–51×8–12 μm, (x̅=44.7×10.5 μm, n=25), obliquely uniseriate to 2–3–seriate, fusiform, slightly curved with narrowly rounded ends, 1–septate, 2–4 guttules in each cell, wall gray while in the ascus, smooth or slightly verruculose. Sexual morph: Not observed.

Culture characteristics – Colonies on PDA reaching 43 mm in 20 days at 25 °C, flat, filiform, gray to near–black from center to edge, with moderate aerial mycelium, smooth, irregular; In reverse, gray to pale brown.

Material examined – China, Guizhou Province, Longli, on decaying wood submerged in a freshwater stream, 2 September 2020, Jian Ma, LLSB06 (GZAAS 21–0383, holotype); ex–type living culture, GZCC 21–0669.

GenBank numbers – OM339435 (ITS), OM339432 (LSU).

Notes – In a BLASTn search of NCBI GenBank, the closest match of LSU and ITS sequence data for Lindgomyces guizhouensis is 99.54% and 96.96% similar to L. pseudomadisonensis (KT 2742). The multi–loci phylogenetic analyses of the combined LSU and ITS sequence dataset confirmed the new strain obtained belonging to Lindgomyces, where it is sister to L. griseosporus with 99% ML and 1.00 BYPP support (Fig. 1). Morphologically, L. guizhouensis is also most similar to L. griseosporus in having superficial, black with roughened surface ascomata; a thick peridium; bitunicate, clavate, subsessile asci and fusiform, 1-septate, guttules ascospores. However, our new collection differs from L. griseosporus in having larger ascomata (396–548×466–514 μm vs 240–290×320–350 μm), fragile peridium and smaller asci (110–154×15–28 μm vs 140–180×24–30). Therefore, we introduce Lindgomyces guizhouensis as a new species.

Figure 1 – Lindgomycesguizhouensis (GZAAS 21–0383, holotype). a–c Superficial ascomata on unidentified plant substrate. d Vertical section of an ascoma. e Peridium. f Hamathecium g Asci with hamathecium h–i Asci j–n Ascospores o Germinating ascospore p–q Colony on PDA from above and below. Scale bars: d, e=100 μm, f–o=20 μm