Laccaria rubriporus S. M. Tang, sp. nov.
Index Fungorum number: IF 900128; MycoBank number: MB 900128; facesoffungi number: FoF 13869; Figs. 1, 2
Etymology – The epithet ‘rubriporus’ refers to the red basidioma of this species.
Diagnosis – Laccaria rubriporus is distinguished by its brownish red to deep orange pileus, small basidioma, and fasciation or scattered, a pastel red to greyish red context, a reddish brown stipe, broadly ellipsoid to basidiospores (6.6–9.3×7.4–10 μm), and the presence of clamps.
Macromorphology – Fasciation or scattered on ground in temperate forests of Pinus. Basidioma small, fasciation or scattered. Pileus 5–15 mm diam., hemispherical to paraboloid, brownish red (8C6-8) at centre, but becoming orange (6A6-7) to dark orange (6A8) towards the margin; margin plicate striate, decurved; trama white (1A1), unchanging. Lamellae pastel red (10A4-5) to greyish red (10D4-5). Lamellulae emarginated with decurrent tooth, regular, close. Stipe 17–22×1–2.2 mm, reddish brown (9E5-8), cylindrical, solid, broadly fistulose, fibrillose-striate. Context thin, pastel red (10A4-5) to greyish red (10D4-5).
Micromorphology – Basidia 34–42×4–11 μm, clavate, 2-spored, rarely 1-spored, sterigmata 5–7 μm long. Basidiospores [113/9/1] (5.9–) 6.6–9.3 (–9.8) × (5.9–) 7.4–10.0 (–10.4) μm, Q = (1.00–) 1.02–1.09 (–1.11), Qm =1.05±0.02, globose, hyaline, echinulate; echinulate 0.7–2.2 μm in length, 0.4–1.0 μm wide at base. Cheilocystidia 14–48×2–4 μm, subclavate, sometimes irregular, thin-walled, hyaline. Pleurocystidia 16–22×3–5 μm, subclavate, thin-walled, hyaline hyphae. Lamella edge appearing as a sterile strip composed of clavate, cylindrical inflated cells 23–34×5–9 μm, thin-walled, colourless, similar to basidioles in shape. Subhymenium 16–24 μm thick, with 2–3-layered, with ovoid, subglobose to ellipsoid or irregular cells of 3–10×3–5 μm. Pileipellis 140–210 μm thick composed of parallelly, thin-walled, cylindrical, greyish-red to reddishorange, filamentous hyphae 9–16 μm thick. Stipe trama composed of longitudinally arranged, clavate terminal cells, light brown, 16–53×3–6 μm. Stipitipellis a cutis, of closely packed, predominantly longitudinally arranged hyphae, with grey pigmentation; hyphae 4–6 μm broad. Clamp present.
Material examined – China, Yunnan Province, Chuxiong Prefecture, Wuding County, 24° 58.289′ N, 100° 48.153′ E, 2078 m a.s.l., 24 July 2019, Song-Ming Tang, T89 (HKAS107552, holotype).
GenBank numbers – ITS: MT500503; LSU: MT500543.
Notes – Our laccaria-like collection is grouped with L. canaliculata, L. glabripes and L. ohiensis (Figs. 1, 2). The ITS locus of our collection compared with these three species and L. canaliculata, L. glabripes and L. ohiensis have 1.95%, 1.3%, and 3.25% nucleotides difference respectively. Further, our collection morphologically differs from these phylogenetically related species.
Figure 1 – Laccaria rubriporus (HKAS107552, holotype). a Basidioma. b OM basidiospores. c–d SEM basidiospores. Scale bars: a=1 cm, b=10 μm, c–d=5 μm
Figure 2 – Laccaria rubriporus. a Stipitipellis. b Hymenium. c Pileipellis. d Basidiospores. e Cheilocystidia. Scale bars: a–c=20 μm, b, d–e=10 μm